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Anti-CDCP1 Antibody (CAB8254)

SKU:
CAB8254
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 12 Anti-CDCP1 Antibody CAB8254
  • Cell Biology Antibodies 12 Anti-CDCP1 Antibody CAB8254
€139 - €419
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Description

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Antibody Name:CDCP1 Rabbit Polyclonal Antibody
Antibody SKU:CAB8254
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-343 of human CDCP1 (NP_835488.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:BT-474, 22Rv1, mouse liver, mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-343 of human CDCP1 (NP_835488.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FEIA LPRE SNIT VLIK LGTP TLLA KPCY IVIS KRHI TMLS IKSG ERIV FTFS CQSP ENHF VIEI QKNI DCMS GPCP FGEV QLQP STSL LPTL NRTF IWDV KAHK SIGL ELQF SIPR LRQI GPGE SCPD GVTH SISG RIDA TVVR IGTF CSNG TVSR IKMQ EGVK MALH LPWF HPRN VSGF SIAN RSSI KRLC IIES VFEG EGSA TLMS ANYP EGFP EDEL MTWQ FVVP AHLR ASVS FLNF NLSN CERK EERV EYYI PGST TNPE VFKL EDKQ PGNM AGNF NLSL QGCD QDAQ SPGI LRLQ FQVL VQHP QNES SE
Gene ID:64866
Uniprot:Q9H5V8
Cellular Location:Cell membrane, Secreted, Single-pass membrane protein
Calculated MW:37kDa/72kDa/92kDa
Observed MW:92kDa/150kDa
Synonyms:CDCP1, CD318, SIMA135, TRASK
Background:This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene.
UniProt Protein Function:CDCP1: a transmembrane protein containing three extracellular CUB domains involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation depending upon its phosphorylation status. Interacts with N- and P-cadherin, syndecans 1 and 4, the serine protease ST14, SRC and PKCG. Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Its expression level is correlated with the metastatic ability of carcinoma cells. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. Three alternatively spliced human isoforms have been reported. Isoform 1 is a single-pass membrane protein that may also be shed, giving rise to a soluble peptide. Isoform 3 is secreted.
UniProt Protein Details:

Protein type:Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Molecular Function:protein binding

NCBI Summary:This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
UniProt Code:Q9H5V8
NCBI GenInfo Identifier:317373455
NCBI Gene ID:64866
NCBI Accession:Q9H5V8.3
UniProt Secondary Accession:Q9H5V8,Q49UB4, Q6NT71, Q6U9Y2, Q8WU91, Q96QU7, Q9H676 Q9H8C2,
UniProt Related Accession:Q9H5V8
Molecular Weight:37,818 Da
NCBI Full Name:CUB domain-containing protein 1
NCBI Synonym Full Names:CUB domain containing protein 1
NCBI Official Symbol:CDCP1  
NCBI Official Synonym Symbols:CD318; TRASK; SIMA135  
NCBI Protein Information:CUB domain-containing protein 1
UniProt Protein Name:CUB domain-containing protein 1
UniProt Synonym Protein Names:Membrane glycoprotein gp140; Subtractive immunization M plus HEp3-associated 135 kDa protein; SIMA135; Transmembrane and associated with src kinases; CD_antigen: CD318
Protein Family:CUB domain-containing protein
UniProt Gene Name:CDCP1  
UniProt Entry Name:CDCP1_HUMAN
Anti-CDCP1 Polyclonal Antibody (CAB8254)Western blot analysis of extracts of various cell lines, using CDCP1 antibody (CAB8254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CDCP1 Polyclonal Antibody (CAB8254)Western blot analysis of extracts of various cell lines, using CDCP1 antibody (CAB8254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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