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Anti-CDK5RAP2 Antibody (CAB15476)

SKU:
CAB15476
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
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Description

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Antibody Name:Anti-CDK5RAP2 Antibody
Antibody SKU:CAB15476
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1744-1893 of human CDK5RAP2 (NP_060719.4).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:LO2, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1744-1893 of human CDK5RAP2 (NP_060719.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GQRL LAEM DIQT QEAP SSTS QELG TKGP HPAP LSKF VSSV STAK LTLE EAYR RLKL LWRV SLPE DGQC PLHC EQIG EMKA EVTK LHKK LFEQ EKKL QNTM KLLQ LSKR QEKV IFDQ LVVT HKIL RKAR GNLE LRPG GAHP GTCS PSRP GS
Gene ID:55755
Uniprot:Q96SN8
Cellular Location:Cytoplasm, Golgi apparatus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:206kDa/210kDa/211kDa/215kDa
Observed MW:250kDa
Synonyms:CDK5RAP2, C48, Cep215, MCPH3
Background:This gene encodes a regulator of CDK5 (cyclin-dependent kinase 5) activity. The protein encoded by this gene is localized to the centrosome and Golgi complex, interacts with CDK5R1 and pericentrin (PCNT), plays a role in centriole engagement and microtubule nucleation, and has been linked to primary microcephaly and Alzheimer's disease. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:CDK5RAP2: Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation. Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3). A disorder defined as a head circumference more than 3 standard deviations below the age- related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 9q33.2

Cellular Component: Golgi apparatus; spindle pole; centrosome; microtubule; pericentriolar material; cytoskeleton; perinuclear region of cytoplasm; cytoplasm; cell junction; cytosol

Molecular Function:calmodulin binding; tubulin binding; protein binding; microtubule binding; protein kinase binding

Biological Process: regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; organelle organization and biogenesis; microtubule cytoskeleton organization and biogenesis; negative regulation of centriole replication; chromosome segregation; negative regulation of neuron differentiation; establishment of mitotic spindle orientation; neurogenesis; centrosome organization and biogenesis; mitotic cell cycle; brain development; G2/M transition of mitotic cell cycle; microtubule bundle formation

Disease: Microcephaly 3, Primary, Autosomal Recessive

NCBI Summary:This gene encodes a regulator of CDK5 (cyclin-dependent kinase 5) activity. The protein encoded by this gene is localized to the centrosome and Golgi complex, interacts with CDK5R1 and pericentrin (PCNT), plays a role in centriole engagement and microtubule nucleation, and has been linked to primary microcephaly and Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
UniProt Code:Q96SN8
NCBI GenInfo Identifier:58535451
NCBI Gene ID:55755
NCBI Accession:NP_060719.4
UniProt Related Accession:Q96SN8
Molecular Weight:
NCBI Full Name:CDK5 regulatory subunit-associated protein 2 isoform a
NCBI Synonym Full Names:CDK5 regulatory subunit associated protein 2
NCBI Official Symbol:CDK5RAP2  
NCBI Official Synonym Symbols:C48; MCPH3; Cep215  
NCBI Protein Information:CDK5 regulatory subunit-associated protein 2
UniProt Protein Name:CDK5 regulatory subunit-associated protein 2
UniProt Synonym Protein Names:CDK5 activator-binding protein C48; Centrosome-associated protein 215
Protein Family:CDK5 regulatory subunit-associated protein
UniProt Gene Name:CDK5RAP2  
UniProt Entry Name:CK5P2_HUMAN
Anti-CDK5RAP2 Antibody (CAB15476)Western blot analysis of extracts of various cell lines, using CDK5RAP2 Rabbit pAb (CAB15476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

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