The C/EBPB Antibody (CAB0711) is a high-quality antibody developed for reliable detection and analysis of target proteins. This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. RRID AB_2757357 Gene ID 1051 Swiss Prot Synonym TCF5; IL6DBP; NF-IL6; C/EBP-beta; C/EBPB
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
C/EBPB Antibody
SKU:
CAB0711
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TCF5, IL6DBP, NF-IL6, C/EBP-beta, C/EBPB
Positive Sample:
SK-MEL-28, HeLa, NIH/3T3
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
36kDa
Observed MW:
48kDa
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. RRID AB_2757357 Gene ID 1051 Swiss Prot Synonym TCF5; IL6DBP; NF-IL6; C/EBP-beta; C/EBPB
Purification Method:
Affinity purification
Gene ID:
1051
RRID:
AB_2757357
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using C/EBPB Rabbit pAb (CAB0711) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using C/EBPB Rabbit pAb (CAB0711) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
