Anti-Centrin-3 Antibody (CAB8111)

Antibody Name:	CETN3 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8111
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human CETN3 (NP_004356.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Jurkat, 293T, Mouse testis, Mouse spleen, Mouse lung, Mouse ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human CETN3 (NP_004356.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLA LRSE LVVD KTKR KKRR ELSE EQKQ EIKD AFEL FDTD KDEA IDYH ELKV AMRA LGFD VKKA DVLK ILKD YDRE ATGK ITFE DFNE VVTD WILE RDPH EEIL KAFK LFDD DDSG KISL RNLR RVAR ELGE NMSD EELR AMIE EFDK DGDG EINQ EEFI AIMT GDI
Gene ID:	1070
Uniprot:	O15182
Cellular Location:	Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus
Calculated MW:	19kDa
Observed MW:	20kDa

Synonyms:

CETN3, CDC31, CEN3, centrin-3

Background:

The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CETN3: Plays a fundamental role in microtubule-organizing center structure and function. Belongs to the centrin family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 5q14.3 Cellular Component: centriole; centrosome; nucleolus Molecular Function:calcium ion binding; protein binding Biological Process: cell division; centrosome cycle; mitosis
NCBI Summary:	The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
UniProt Code:	O15182
NCBI GenInfo Identifier:	85567504
NCBI Gene ID:	1070
NCBI Accession:	AAI12041.1
UniProt Secondary Accession:	O15182,Q53YD2, Q9BS23,
UniProt Related Accession:	O15182
Molecular Weight:	19,550 Da
NCBI Full Name:	Centrin 3
NCBI Synonym Full Names:	centrin 3
NCBI Official Symbol:	CETN3
NCBI Official Synonym Symbols:	CEN3; CDC31
NCBI Protein Information:	centrin-3
UniProt Protein Name:	Centrin-3
Protein Family:	Centrin
UniProt Gene Name:	CETN3
UniProt Entry Name:	CETN3_HUMAN

	Western blot analysis of extracts of various cell lines, using CETN3 antibody (CAB8111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human lung cancer using CETN3 antibody (CAB8111) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using CETN3 antibody (CAB8111) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using CETN3 antibody (CAB8111) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using CETN3 antibody (CAB18111) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

CETN3 Antibody (PACO08378)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)