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Anti-CNBP Antibody (CAB15110)

SKU:
CAB15110
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-CNBP Antibody CAB15110
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-CNBP Antibody CAB15110
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Description

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Antibody Name:Anti-CNBP Antibody
Antibody SKU:CAB15110
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human CNBP (NP_001120668.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, A-549, LO2, mouse brain, mouse kidney, mouse liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human CNBP (NP_001120668.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LARD CDHA DEQK CYSC GEFG HIQK DCTK VKCY RCGE TGHV AINC SKTS EVNC YRCG ESGH LARE CTIE ATA
Gene ID:7555
Uniprot:P62633
Cellular Location:Cytoplasm, Endoplasmic reticulum
Calculated MW:17kDa/18kDa/19kDa
Observed MW:19kDa
Synonyms:CNBP, CNBP1, DM2, PROMM, RNF163, ZCCHC22, ZNF9
Background:This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion from <30 repeats to 75-11000 repeats in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:ZNF9: Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression. Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2); also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA-binding; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: cytosol; endoplasmic reticulum; nucleus

Molecular Function:protein binding; transcription factor activity

Biological Process: cholesterol biosynthetic process; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent

Disease: Myotonic Dystrophy 2

NCBI Summary:This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion from <30 repeats to 75-11000 repeats in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016]
UniProt Code:P62633
NCBI GenInfo Identifier:50401852
NCBI Gene ID:7555
NCBI Accession:P62633.1
UniProt Secondary Accession:P62633,P20694, Q4JGY0, Q4JGY1, Q5QJR0, A8K7V4, B2RAV9 B4DP17, D3DNB9, D3DNC0, D3DNC1, E9PDR7,
UniProt Related Accession:P62633
Molecular Weight:18,871 Da
NCBI Full Name:Cellular nucleic acid-binding protein
NCBI Synonym Full Names:CCHC-type zinc finger nucleic acid binding protein
NCBI Official Symbol:CNBP  
NCBI Official Synonym Symbols:DM2; ZNF9; CNBP1; PROMM; RNF163; ZCCHC22  
NCBI Protein Information:cellular nucleic acid-binding protein
UniProt Protein Name:Cellular nucleic acid-binding protein
UniProt Synonym Protein Names:Zinc finger protein 9
Protein Family:Cellular nucleic acid-binding protein
UniProt Gene Name:CNBP  
UniProt Entry Name:CNBP_HUMAN
Anti-CNBP Antibody (CAB15110)Western blot analysis of extracts of various cell lines, using CNBP antibody (CAB15110) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-CNBP Antibody (CAB15110)Immunofluorescence analysis of U-2 OS cells using CNBP antibody (CAB15110) at dilution of 1:100. Blue: DAPI for nuclear staining.

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