The CPSF3L Antibody (CAB6566) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By targeting the CPSF3L protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for studies in molecular biology and gene regulation.CPSF3L is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, which plays a crucial role in post-transcriptional mRNA processing.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CPSF3L Antibody
SKU:
CAB6566
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RC68, INT11, RC-68, CPSF3L, CPSF73L
Positive Sample:
HeLa, SKOV3, MCF7, Mouse spleen, Rat liver
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
68kDa
Observed MW:
68kDa
The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex (Baillat et al., 2005 [PubMed 16239144]).
Purification Method
Affinity purification
Gene ID
54973
RRID
AB_2767160
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CPSF3L Rabbit pAb (CAB6566) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using CPSF3L Rabbit pAb (CAB6566). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
