Anti-CRKII Antibody (CAB9577)

SKU:
CAB9577
€129 - €349

Description

Technical Manual

Anti-CRKII Antibody

Product Name:Anti-CRKII Antibody
Product SKU:CAB9577
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008]

Immunogen:A synthesized peptide derived from human CRKII
Sequence:

Email for sequence

Gene ID:1398
Swiss Prot:P46108
Synonyms:CRKII; p38
Calculated MW:38kDa
Observed MW:37KDa
Reactivity:Human, Mouse
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:293T, A-549, Mouse lung
Cellular Location:

Product Images

Anti-CRKII Antibody (CAB9577)

Western blot - CRKII Rabbit mAb (CAB9577)

Uniprot Information

UniProt Protein Function:CRK: an adaptor protein with an SH2-SH3-SH3 domain structure. Recruits cytoplasmic proteins through SH2-phospho-tyrosine interaction. Phosphorylated by Abl, IGF-IR and EGFR. Phosphorylation induces a change in intramolecular folding and SH2 interactions, causing its rapid dissociation from the tyrosine kinase complex.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold; Oncoprotein

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cytoplasm; plasma membrane; cytosol; nucleus; actin cytoskeleton

Molecular Function:protein phosphorylated amino acid binding; protein binding; ephrin receptor binding; SH3/SH2 adaptor activity; SH2 domain binding

Biological Process: regulation of transcription from RNA polymerase II promoter; regulation of Rac protein signal transduction; platelet activation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; regulation of actin cytoskeleton organization and biogenesis; insulin receptor signaling pathway; ephrin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation

NCBI Summary:This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008]
UniProt Code:P46108
NCBI GenInfo Identifier:158939322
NCBI Gene ID:1398
NCBI Accession:P46108.2
UniProt Secondary Accession:P46108,Q96GA9, Q96HJ0, A8MWE8, B0LPE8, D3DTH6,
UniProt Related Accession:P46108
Molecular Weight:304
NCBI Full Name:Adapter molecule crk
NCBI Synonym Full Names:v-crk avian sarcoma virus CT10 oncogene homolog
NCBI Official Symbol:CRK
NCBI Official Synonym Symbols:p38; CRKII
NCBI Protein Information:adapter molecule crk; proto-oncogene c-Crk
UniProt Protein Name:Adapter molecule crk
UniProt Synonym Protein Names:Proto-oncogene c-Crk; p38
Protein Family:Crk-like protein
UniProt Gene Name:CRK
UniProt Entry Name:CRK_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
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