The CRK/CRKII Monoclonal Antibody (CAB9577) is a high-quality antibody developed for reliable detection and analysis of target proteins. This highly specific antibody, generated in rabbits, is suitable for use in Western blotting, immunoprecipitation, and immunofluorescence assays, allowing for the detection and analysis of CRKII in different cell types.CRKII is a key player in signal transduction pathways that control cell growth and differentiation, making it a target of interest in cancer research and developmental biology. Its role in promoting cell adhesion and migration also implicates it in diseases such as metastasis and invasion.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CRK/CRKII Monoclonal Antibody
SKU:
CAB9577
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1644
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p38, CRKII, CRK/CRKII
Positive Sample:
293T, A549, Mouse lung
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
34kDa
Observed MW:
37kDa
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
Purification Method
Affinity purification
Gene ID
1398
RRID
AB_2863729
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CRK/CRK/CRKII Rabbit mAb (CAB9577) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
