Anti-CTRB1 Antibody (CAB15043)

Antibody Name:	Anti-CTRB1 Antibody
Antibody SKU:	CAB15043
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 79-263 of human CTRB1 (NP_001897.4).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse spleen, Mouse stomach

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 79-263 of human CTRB1 (NP_001897.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RTSD VVVA GEFD QGSD EENI QVLK IAKV FKNP KFSI LTVN NDIT LLKL ATPA RFSQ TVSA VCLP SADD DFPA GTLC ATTG WGKT KYNA NKTP DKLQ QAAL PLLS NAEC KKSW GRRI TDVM ICAG ASGV SSCM GDSG GPLV CQKD GAWT LVGI VSWG SDTC STSS PGVY ARVT KLIP WVQK ILAA N
Gene ID:	1504
Uniprot:	P17538
Cellular Location:	Secreted, extracellular space
Calculated MW:	27kDa
Observed MW:	28kDa

Synonyms:

CTRB1, CTRB

Background:

This gene encodes a member of the serine protease family of enzymes and forms a principal precursor of the pancreatic proteolytic enzymes. The encoded preproprotein is synthesized in the acinar cells of the pancreas and secreted into the small intestine where it undergoes proteolytic activation to generate a functional enzyme. This gene is located adjacent to a related chymotrypsinogen gene. This gene encodes distinct isoforms, some or all of which may undergo similar processing to generate the mature protein.

UniProt Protein Function:	CTRB1: is one of a family of serine proteases that is secreted into the gastrointestinal tract as an inactive precursor, which is activated by proteolytic cleavage with trypsin. [provided by RefSeq, Oct 2011]
UniProt Protein Details:	Protein type:EC 3.4.21.1; Secreted, signal peptide; Protease; Secreted; Apoptosis Chromosomal Location of Human Ortholog: 16q23.1 Cellular Component: extracellular region; extracellular space Molecular Function:serine-type endopeptidase activity Biological Process: cobalamin metabolic process; digestion; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis; vitamin metabolic process; water-soluble vitamin metabolic process
NCBI Summary:	The protein encoded by this gene is one of a family of serine proteases that is secreted into the gastrointestinal tract as an inactive precursor, which is activated by proteolytic cleavage with trypsin. [provided by RefSeq, Oct 2011]
UniProt Code:	P17538
NCBI GenInfo Identifier:	117617
NCBI Gene ID:	1504
NCBI Accession:	P17538.1
UniProt Related Accession:	P17538
Molecular Weight:	27,870 Da
NCBI Full Name:	Chymotrypsinogen B
NCBI Synonym Full Names:	chymotrypsinogen B1
NCBI Official Symbol:	CTRB1
NCBI Official Synonym Symbols:	CTRB
NCBI Protein Information:	chymotrypsinogen B
UniProt Protein Name:	Chymotrypsinogen B
Protein Family:	Chymotrypsinogen
UniProt Gene Name:	CTRB1
UniProt Entry Name:	CTRB1_HUMAN

	Western blot analysis of extracts of various cell lines, using CTRB1 antibody (CAB15043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded mouse lung using CTRB1 antibody (CAB15043) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse pancreas using CTRB1 antibody (CAB15043) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of rat pancreas using CTRB1 Polyclonal Antibody (CAB15043) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse pancreas using CTRB1 Polyclonal Antibody (CAB15043) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)