Anti-CTSG Antibody (CAB13172)

Antibody Name:	Anti-CTSG Antibody
Antibody SKU:	CAB13172
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 75-190 of human CTSG (NP_001902.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 75-190 of human CTSG (NP_001902.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GAHN IQRR ENTQ QHIT ARRA IRHP QYNQ RTIQ NDIM LLQL SRRV RRNR NVNP VALP RAQE GLRP GTLC TVAG WGRV SMRR GTDT LREV QLRV QRDR QCLR IFGS YDPR RQIC VGDR
Gene ID:	1511
Uniprot:	P08311
Cellular Location:	Cell surface
Calculated MW:	28kDa
Observed MW:	29kDa

Synonyms:

CTSG, CATG, CG

Background:

The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene.

UniProt Protein Function:	CTSG: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe- CH2Cl and phenylmethylsulfonyl fluoride. Belongs to the peptidase S1 family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; EC 3.4.21.20; Protease Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: extracellular matrix; extracellular space; cell surface; extracellular region; plasma membrane; nucleus; secretory granule Molecular Function:heparin binding; peptidase activity; serine-type endopeptidase activity Biological Process: extracellular matrix disassembly; positive regulation of immune response; extracellular matrix organization and biogenesis; cellular protein metabolic process; response to lipopolysaccharide; immune response; protein processing; proteolysis; angiotensin maturation; defense response to fungus
NCBI Summary:	The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014]
UniProt Code:	P08311
NCBI GenInfo Identifier:	115725
NCBI Gene ID:	1511
NCBI Accession:	P08311.2
UniProt Secondary Accession:	P08311,Q6IBJ6, Q9UCA5, Q9UCU6,
UniProt Related Accession:	P08311
Molecular Weight:	255
NCBI Full Name:	Cathepsin G
NCBI Synonym Full Names:	cathepsin G
NCBI Official Symbol:	CTSG
NCBI Official Synonym Symbols:	CG; CATG
NCBI Protein Information:	cathepsin G
UniProt Protein Name:	Cathepsin G
Protein Family:	Cathepsin
UniProt Gene Name:	CTSG
UniProt Entry Name:	CATG_HUMAN

	Western blot analysis of extracts of various cell lines, using CTSG antibody (CAB13172) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human tonsil using CTSG antibody (CAB13172) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using CTSG antibody (CAB13172) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human uterine cancer using CTSG antibody (CAB13172) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using CTSG antibody (CAB13172) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-CTSG Antibody (CAB2679)	Human Cathepsin G ELISA Kit
Anti-CTSG Antibody (CAB5636)	Human Cathepsin G (CTSG) ELISA Kit
Cleaved-CTSG (I21) Antibody (PACO00023)
CTSG Antibody (PACO02524)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)