Anti-Cytochrome c Antibody (CAB13430)

Antibody Name:	Anti-Cytochrome c Antibody [KO Validated]
Antibody SKU:	CAB13430
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, SH-SY5Y, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGDV EKGK KIFI MKCS QCHT VEKG GKHK TGPN LHGL FGRK TGQA PGYS YTAA NKNK GIIW GEDT LMEY LENP KKYI PGTK MIFV GIKK KEER ADLI AYLK KATN E
Gene ID:	54205
Uniprot:	P99999
Cellular Location:	Mitochondrion intermembrane space
Calculated MW:	11kDa
Observed MW:	11KDa

Synonyms:

CYCS, CYC, HCS, THC4, cytochrome c

Background:

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.

UniProt Protein Function:	CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family.
UniProt Protein Details:	Protein type:Apoptosis; Oxidoreductase; Mitochondrial; Protein phosphatase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 7p15.3 Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrion; nucleus; protein phosphatase type 2A complex Molecular Function:electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; heme binding; protein binding; protein serine/threonine phosphatase activity Biological Process: caspase activation via cytochrome c; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; mitochondrial electron transport, ubiquinol to cytochrome c; mitochondrion organization and biogenesis; response to reactive oxygen species Disease: Thrombocytopenia 4
NCBI Summary:	This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
UniProt Code:	P99999
NCBI GenInfo Identifier:	42560196
NCBI Gene ID:	54205
NCBI Accession:	P99999.2
UniProt Secondary Accession:	P99999,P00001, Q6NUR2, Q6NX69, Q96BV4, A4D166, B2R4I1
UniProt Related Accession:	P99999
Molecular Weight:	11,749 Da
NCBI Full Name:	Cytochrome c
NCBI Synonym Full Names:	cytochrome c, somatic
NCBI Official Symbol:	CYCS
NCBI Official Synonym Symbols:	CYC; HCS; THC4
NCBI Protein Information:	cytochrome c
UniProt Protein Name:	Cytochrome c
Protein Family:	Cytochrome
UniProt Gene Name:	CYCS
UniProt Entry Name:	CYC_HUMAN

	Western blot analysis of extracts of various cell lines, using Cytochrome c Rabbit pAb (CAB13430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts from normal (control) and Cytochrome c knockout (KO) 293T cells, using Cytochrome c antibody (CAB13430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using Cytochrome c antibody (CAB13430) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human tonsil using Cytochrome c antibody (CAB13430) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using Cytochrome c antibody (CAB13430) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-Cytochrome c Antibody (CAB0225)	Human Cytochrome c ELISA Kit
Anti-Cytochrome C [KO Validated] Antibody (CAB4912)	Human Cyt-C (Cytochrome C) CLIA Kit (HUES00054)
CYCS Antibody (PACO00659)	Human Cyt-C (Cytochrome C) ELISA Kit (HUES01343)
CYCS Antibody (PACO07483)	Human Cytochrome c (CYCS) ELISA Kit
CYCS Antibody (PACO08701)	Cytochrome c Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)