The [KO Validated] Cytochrome C Antibody (CAB13430) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific to human samples, making it ideal for various research applications, including Western blotting. By binding to cytochrome c, this antibody enables precise detection and analysis in a range of cell types, offering valuable insights into the mechanisms of cell death and survival.Cytochrome c is a key player in the intrinsic pathway of apoptosis, where it is released from mitochondria into the cytoplasm to trigger a series of events leading to cell death.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] Cytochrome C Antibody
SKU:
CAB13430
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
MGDV EKGK KIFI MKCS QCHT VEKG GKHK TGPN LHGL FGRK TGQA PGYS YTAA NKNK GIIW GEDT LMEY LENP KKYI PGTK MIFV GIKK KEER ADLI AYLK KATN E
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CYC, HCS, THC4, Cytochrome C
Positive Sample:
Mouse kidney, HeLa, 293T, C2C12, Rat kidney, C6
Cellular Localization:
Mitochondrion Intermembrane Space.
Calculated MW:
12kDa
Observed MW:
14kDa
This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
Purification Method
Affinity purification
Gene ID
54205
RRID
AB_2760292
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse kidney using Cytochrome C Rabbit pAb (CAB13430) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of various lysates using Cytochrome C Rabbit pAb (CAB13430) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat colon using Cytochrome C Rabbit pAb (CAB13430) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of HepG2 cells using Cytochrome C Rabbit pAb (CAB13430) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
