Description
Cytotoxicity Control Reagent (Saponin) (BA0005) (BA0005)
The Cytotoxicity Control Reagent (Saponin) (SKU: BA0005) is a 50 mg preparation of saponin supplied as a positive cytotoxic control for use with cell viability and cytotoxicity assays. Saponin is a detergent that permeabilises cell membranes and reliably induces cell death, making it an ideal reference for total lysis or maximal cytotoxicity controls. The reagent is conveniently reconstituted in phosphate buffered saline to give a 1% saponin stock and is typically used at a final concentration around 0.1%. It is designed to accompany colorimetric and fluorescent cell viability kits, where a dose-dependent cytotoxic response can be demonstrated. Supplying a consistent, well-characterised control reagent supports reliable comparison between assays and plates.
| Product Name: | Cytotoxicity Control Reagent (Saponin) (BA0005) |
| SKU: | BA0005 |
| Detection Method: | N/A (control reagent) |
| Sample Type: | ['Cultured cells'] |
| Species Reactivity: | All |
| Kit Size: | 50 mg |
| Equipment Required: | Microplate reader |
| Storage: | -20°C |
| Shipping: | Room Temperature |
Saponin is a cytotoxic detergent used as a positive control reagent in cell viability and cytotoxicity assays. It permeabilises the cell membrane to induce cell death, providing a total-lysis or maximal-cytotoxicity reference point. The reagent can be conveniently reconstituted with 5 mL phosphate buffered saline to give a 1% saponin solution and is commonly used at a final concentration of approximately 0.1% saponin in the assay well. It has been characterised as a cytotoxic control with an IC50 of approximately 0.002 wt%.
- Reliable positive cytotoxic control for cell viability and cytotoxicity assays.
- Conveniently reconstituted in phosphate buffered saline to give a 1% saponin stock.
- Well characterised, giving a reproducible dose-dependent cytotoxic response.
- Positive cytotoxic control for colorimetric and fluorescent cell viability assays.
- Total-lysis / maximal-cytotoxicity reference control.
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reconstitute the Control Reagent with 5 mL phosphate buffered saline (PBS) to make a 1% saponin solution. |
| 2 | Add the reconstituted control reagent to designated control wells to a final concentration of approximately 0.1% saponin, alongside the test samples in the cell viability or cytotoxicity assay. |
| 3 | Incubate and process the control wells in parallel with the test samples according to the accompanying assay protocol. |
| Component | Quantity | Storage |
| Saponin | 50 mg | -20°C |
