The Delta-sarcoglycan (SGCD) Antibody (CAB6980) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting Delta Sarcoglycan in human samples, making it ideal for Western blot applications.Delta Sarcoglycan is a member of the sarcoglycan complex, which plays a crucial role in maintaining the structural integrity of muscle cells. Mutations in Delta Sarcoglycan have been linked to various forms of muscular dystrophy, including limb-girdle muscular dystrophy.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Delta-sarcoglycan (SGCD) Antibody
SKU:
CAB6980
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Jurkat, THP-1, Mouse heart, Mouse skeletal muscle, Rat heart, Rat skeletal muscle
Cellular Localization:
Cell Membrane, Cytoplasm, Single-Pass Type Ii Membrane Protein, Cytoskeleton, Sarcolemma.
Calculated MW:
32kDa
Observed MW:
37kDa
The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
6444
RRID
AB_2767536
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Delta-sarcoglycan (SGCD) Rabbit pAb (CAB6980) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat heart using Delta-sarcoglycan (SGCD) Rabbit pAb (CAB6980) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
