Anti-SGCD Antibody (CAB6980)

Antibody Name:	Anti-SGCD Antibody
Antibody SKU:	CAB6980
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 61-290 of human SGCD (NP_000328.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, THP-1, Mouse heart, Mouse skeletal muscle, Rat heart, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 61-290 of human SGCD (NP_000328.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NFTI DGMG NLRI TEKG LKLE GDSE FLQP LYAK EIQS RPGN ALYF KSAR NVTV NILN DQTK VLTQ LITG PKAV EAYG KKFE VKTV SGKL LFSA DNNE VVVG AERL RVLG AEGT VFPK SIET PNVR ADPF KELR LESP TRSL VMEA PKGV EINA EAGN MEAT CRTE LRLE SKDG EIKL DAAK IRLP RLPH GSYT PTGT RQKV FEIC VCAN GRLF LSQA GAGS TCQI NTSV CL
Gene ID:	6444
Uniprot:	Q92629
Cellular Location:	Cell membrane, Cytoplasm, Single-pass type II membrane protein, cytoskeleton, sarcolemma
Calculated MW:	28kDa/32kDa
Observed MW:	37kDa

Synonyms:

SGCD, 35DAG, CMD1L, DAGD, SG-delta, SGCDP, SGD

Background:

The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.

UniProt Protein Function:	SGCD: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Defects in SGCD are the cause of limb-girdle muscular dystrophy type 2F (LGMD2F). LGMD2F is an autosomal recessive disorder. Defects in SGCD are the cause of cardiomyopathy dilated type 1L (CMD1L). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the sarcoglycan beta/delta/gamma/zeta family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Dystrophin complex; Membrane protein, integral Chromosomal Location of Human Ortholog: 5q33-q34 Cellular Component: dystrophin-associated glycoprotein complex; cytoskeleton; cytoplasm; integral to membrane; plasma membrane; sarcoglycan complex; sarcolemma Biological Process: cardiac muscle development; muscle development; heart contraction; muscle cell development Disease: Muscular Dystrophy, Limb-girdle, Type 2f; Cardiomyopathy, Dilated, 1l
NCBI Summary:	The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92629
NCBI GenInfo Identifier:	212276471
NCBI Gene ID:	6444
NCBI Accession:	Q92629.2
UniProt Secondary Accession:	Q92629,Q53XA5, Q99644, A8K9S9,
UniProt Related Accession:	Q92629
Molecular Weight:	289
NCBI Full Name:	Delta-sarcoglycan
NCBI Synonym Full Names:	sarcoglycan, delta (35kDa dystrophin-associated glycoprotein)
NCBI Official Symbol:	SGCD
NCBI Official Synonym Symbols:	SGD; DAGD; 35DAG; CMD1L; SGCDP; SG-delta
NCBI Protein Information:	delta-sarcoglycan; delta-SG; placental delta sarcoglycan; 35 kDa dystrophin-associated glycoprotein; dystrophin associated glycoprotein, delta sarcoglycan
UniProt Protein Name:	Delta-sarcoglycan
UniProt Synonym Protein Names:	35 kDa dystrophin-associated glycoprotein; 35DAG
Protein Family:	Delta-sarcoglycan
UniProt Gene Name:	SGCD
UniProt Entry Name:	SGCD_HUMAN

	Western blot analysis of extracts of various cell lines, using SGCD antibody (CAB6980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat heart using SGCD antibody (CAB6980) at dilution of 1:100 (40x lens).

Antibodies

Anti-SGCD Antibody (CAB13351)

Anti-delta Sarcoglycan Antibody (CAB9175)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)