Anti-DENR Antibody (CAB13699)

Antibody Name:	Anti-DENR Antibody
Antibody SKU:	CAB13699
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human DENR (NP_003668.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, BxPC-3, HeLa, Jurkat, Mouse heart, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human DENR (NP_003668.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAD ISES SGAD CKGD PRNS AKLD ADYP LRVL YCGV CSLP TEYC EYMP DVAK CRQW LEKN FPNE FAKL TVEN SPKQ EAGI SEGQ GTAG EEEE KKKQ KRGG RGQI KQKK KTVP QKVT IAKI PRAK KKYV TRVC GLAT FEID LKEA QRFF AQKF SCGA SVTG EDEI IIQG DFTD DIID VIQE KWPE VDDD SIED LGEV KK
Gene ID:	8562
Uniprot:	O43583
Cellular Location:
Calculated MW:	22kDa
Observed MW:	30kDa

Synonyms:

DENR, DRP, DRP1, SMAP-3

Background:

This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants.

UniProt Protein Function:	DENR: May be involved in the translation of target mRNAs by scanning and recognition of the initiation codon. Plays a role in the modulation of the translational profile of a subset of cancer- related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1. Belongs to the DENR family.
UniProt Protein Details:	Protein type:Translation; RNA-binding Chromosomal Location of Human Ortholog: 12q24.31 Molecular Function:protein binding; translation initiation factor activity Biological Process: ribosome disassembly; formation of translation preinitiation complex
NCBI Summary:	This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants. [provided by RefSeq, Jul 2008]
UniProt Code:	O43583
NCBI GenInfo Identifier:	13637727
NCBI Gene ID:	8562
NCBI Accession:	O43583.2
UniProt Secondary Accession:	O43583,Q9H3U6, Q9UKZ0,
UniProt Related Accession:	O43583
Molecular Weight:	198
NCBI Full Name:	Density-regulated protein
NCBI Synonym Full Names:	density-regulated protein
NCBI Official Symbol:	DENR
NCBI Official Synonym Symbols:	DRP; DRP1; SMAP-3
NCBI Protein Information:	density-regulated protein; smooth muscle cell associated protein-3; smooth muscle cell-associated protein 3
UniProt Protein Name:	Density-regulated protein
UniProt Synonym Protein Names:	Protein DRP1; Smooth muscle cell-associated protein 3; SMAP-3
Protein Family:	Density-regulated protein
UniProt Gene Name:	DENR
UniProt Entry Name:	DENR_HUMAN

	Western blot analysis of extracts of various cell lines, using DENR antibody (CAB13699) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat heart using DENR antibody (CAB13699) at dilution of 1:150 (40x lens).

Antibodies

DENR Antibody (PACO08806)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)