The Desmin Antibody (CAB1116) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human DES samples and is validated for use in immunohistochemistry and immunofluorescence applications. It binds specifically to the DES protein, allowing for detection and visualization in different cell types, making it an essential tool for studies in cell biology and cancer research.DES, also known as desmin, is a key component of the cytoskeleton in muscle cells and plays a crucial role in maintaining cell structure and function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Desmin Antibody
SKU:
CAB1116
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CSM1, CSM2, CDCD3, LGMD1D, LGMD1E, LGMD2R, Desmin
Positive Sample:
C2C12
Cellular Localization:
Cell Membrane, Cytoplasm, Sarcolemma.
Calculated MW:
54kDa
Observed MW:
55kDa
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
Purification Method
Affinity purification
Gene ID
1674
RRID
AB_2758437
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C2C12 cells, using Desmin Rabbit pAb (CAB1116) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
