The Desmin Monoclonal Antibody (CAB3736) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. RRID AB_2863132 Gene ID 1674 Swiss Prot Synonym CSM1; CSM2; CDCD3; LGMD1D; LGMD1E; LGMD2R; Desmin
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Desmin Monoclonal Antibody
SKU:
CAB3736
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0235
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAIF-P
Recommended Dilution:
WB
1:1000 - 1:5000
IF-P
1:100 - 1:1000
IHC-P
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CSM1, CSM2, CDCD3, LGMD1D, LGMD1E, LGMD2R, Desmin
Positive Sample:
C2C12, RD, Mouse lung, Mouse heart, Rat heart
Cellular Localization:
Cell Membrane, Cytoplasm, Sarcolemma.
Calculated MW:
54kDa
Observed MW:
54kDa
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. RRID AB_2863132 Gene ID 1674 Swiss Prot Synonym CSM1; CSM2; CDCD3; LGMD1D; LGMD1E; LGMD2R; Desmin
Purification Method:
Affinity purification
Gene ID:
1674
RRID:
AB_2863132
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Desmin Rabbit mAb (CAB3736) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of paraffin-embedded mouse heart using Desmin Rabbit mAb (CAB3736) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
