The Desmoglein 3 Antibody (CAB8412) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot and immunohistochemistry. By specifically binding to the Desmoglein-3 protein, this antibody enables precise detection and analysis in different cell types, making it ideal for studies in dermatology, oncology, and autoimmune diseases.Desmoglein-3 is a key component of desmosomes, specialized structures that mediate strong cell-to-cell adhesion in epithelial tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Desmoglein 3 Antibody
SKU:
CAB8412
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PVA, ABOLM, CDHF6, Desmoglein 3
Positive Sample:
A431
Cellular Localization:
Cell Junction, Cell Membrane, Single-Pass Type I Membrane Protein, Desmosome.
Calculated MW:
108kDa
Observed MW:
130kDa
This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris.
Purification Method
Affinity purification
Gene ID
1830
RRID
AB_2769232
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Desmoglein 3 Rabbit pAb (CAB8412) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
