Diamine Oxidase Assay Kit (Fluorometric) (BA0172)

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ELISA Kit Technical ManualMSDS

Diamine Oxidase Assay Kit

DIAMINE OXIDASE (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the digestive tract and placenta. An imbalance between histamine intake and the capacity for histamine degradation can lead to histamine intolerance (HIT). Measuring DAO activity in serum can be useful in diagnosing HIT.

Assay Genie's non-radioactive, fluorimetric Diamine Oxidase Assay (DAO) assay is based on the oxidation of putrescine to pyrroline plus NH3 and H2O2. The generated H2O2 is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at Ex/Em = 530/585 nm is directly proportional to the enzyme activity.


For quantitative determination of diamine oxidase in biological samples (e.g. serum, plasma etc)

Diamine Oxidase Assay Kit - Key Features

  • Fast and sensitive. Use of 10 uL sample. Linear detection range 0.5 to 6 U/L for 30 min reaction at 25°C.
  • Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37°C heater is needed.
  • High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Diamine Oxidase Assay Kit Information:

Kit Includes: Assay Buffer: 10 mL HRP Enzyme: 120 uL Substrate: 120 uL H2O2 Standard: 100 uL Dye Reagent: 120 uL
Kit Requires: Pipetting devices, centrifuge tubes, black flat-bottom 96-well plates and plate reader
Method of Detection: FL530/585nm
Detection Limit: 0.5 U/L
Samples: Serum, Plasma
Species: All
Protocol Length: 30 min
Size: 100 tests
Storage: Store all components at -20°C upon receiving
Shelf Life: 6 months


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