Anti-DLG1 Antibody (CAB8542)

Antibody Name:	Anti-DLG1 Antibody
Antibody SKU:	CAB8542
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human DLG1 (NP_004078.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, LO2, HeLa, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human DLG1 (NP_004078.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPVR KQDT QRAL HLLE EYRS KLSQ TEDR QLRS SIER VINI FQSN LFQA LIDI QEFY EVTL LDNP KCID RSKP SEPI QPVN TWEI SSLP SSTV TSET LPSS LSPS VEKY RYQD EDTP PQEH ISPQ ITNE VIGP ELVH VSEK NLSE IENV HGFV SHSH ISPI KPTE AVLP SPPT VPVI PVLP VPAE NTVI LP
Gene ID:	1739
Uniprot:	Q12959
Cellular Location:	Basolateral cell membrane, Cell junction, Cell membrane, Endoplasmic reticulum membrane, Membrane, Peripheral membrane protein, postsynaptic cell membrane, postsynaptic density, sarcolemma, synapse
Calculated MW:	87-103kDa
Observed MW:	114kDa

Synonyms:

DLG1, DLGH1, SAP-97, SAP97, dJ1061C18.1.1, hdlg

Background:

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.

UniProt Protein Function:	Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.
NCBI Summary:	This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known. [provided by RefSeq, Feb 2011]
UniProt Code:	Q12959
NCBI GenInfo Identifier:	223590196
NCBI Gene ID:	1739
NCBI Accession:	Q12959.2
UniProt Secondary Accession:	Q12959,Q12958, A5YKK7, B4DGU1, B4DGZ8, B7ZMM0, B9EIQ5 D3DXB8, D3DXB9, E7EWL7, E9PG21,
UniProt Related Accession:	Q12959
Molecular Weight:	100kDa
NCBI Full Name:	Disks large homolog 1
NCBI Synonym Full Names:	discs large MAGUK scaffold protein 1
NCBI Official Symbol:	DLG1
NCBI Official Synonym Symbols:	hdlg; DLGH1; SAP97; SAP-97; dJ1061C18.1.1
NCBI Protein Information:	disks large homolog 1
UniProt Protein Name:	Disks large homolog 1
UniProt Synonym Protein Names:	Synapse-associated protein 97; SAP-97; SAP97; hDlg
Protein Family:	Disks large
UniProt Gene Name:	DLG1

	Western blot analysis of extracts of various cell lines, using DLG1 antibody (CAB8542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using DLG1 antibody (CAB8542) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using DLG1 antibody (CAB8542) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using DLG1 antibody (CAB8542) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using DLG1 Rabbit pAb (CAB8542) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-DLG1 Antibody (CAB12432)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)