Anti-DNAJA2 Antibody (CAB7059)

Antibody Name:	Anti-DNAJA2 Antibody
Antibody SKU:	CAB7059
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 233-412 of human DNAJA2 (NP_005871.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-375, 293T, HepG2, MCF7, Mouse kidney, Mouse heart, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 233-412 of human DNAJA2 (NP_005871.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FTGE ADQA PGVE PGDI VLLL QEKE HEVF QRDG NDLH MTYK IGLV EALC GFQF TFKH LDGR QIVV KYPP GKVI EPGC VRVV RGEG MPQY RNPF EKGD LYIK FDVQ FPEN NWIN PDKL SELE DLLP SRPE VPNI IGET EEVE LQEF DSTR GSGG GQRR EAYN DSSD EESS SHHG PGVQ CAHQ
Gene ID:	10294
Uniprot:	O60884
Cellular Location:	Lipid-anchor, Membrane
Calculated MW:	45kDa
Observed MW:	46kDa

Synonyms:

DNAJA2, CPR3, DJ3, DJA2, DNAJ, DNJ3, HIRIP4, PRO3015, RDJ2

Background:

The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.

UniProt Protein Function:	DNAJA2: Co-chaperone of Hsc70.
UniProt Protein Details:	Protein type:Cell cycle regulation; Chaperone Chromosomal Location of Human Ortholog: 16q12.1 Cellular Component: membrane; cytosol Molecular Function:protein binding; heat shock protein binding; metal ion binding; chaperone binding; unfolded protein binding; ATP binding Biological Process: response to heat; positive regulation of cell proliferation; protein refolding
NCBI Summary:	The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq, Jul 2008]
UniProt Code:	O60884
NCBI GenInfo Identifier:	14916548
NCBI Gene ID:	10294
NCBI Accession:	O60884.1
UniProt Secondary Accession:	O60884,O14711, B2R7L7,
UniProt Related Accession:	O60884
Molecular Weight:	412
NCBI Full Name:	DnaJ homolog subfamily A member 2
NCBI Synonym Full Names:	DnaJ (Hsp40) homolog, subfamily A, member 2
NCBI Official Symbol:	DNAJA2
NCBI Official Synonym Symbols:	DJ3; CPR3; DJA2; DNAJ; DNJ3; RDJ2; HIRIP4; PRO3015
NCBI Protein Information:	dnaJ homolog subfamily A member 2; HIRA interacting protein 4; HIRA-interacting protein 4; cell cycle progression 3 protein; renal carcinoma antigen NY-REN-14; cell cycle progression restoration gene 3 protein
UniProt Protein Name:	DnaJ homolog subfamily A member 2
UniProt Synonym Protein Names:	Cell cycle progression restoration gene 3 protein; Dnj3; Dj3; HIRA-interacting protein 4; Renal carcinoma antigen NY-REN-14
Protein Family:	DnaJ homolog subfamily
UniProt Gene Name:	DNAJA2
UniProt Entry Name:	DNJA2_HUMAN

	Western blot analysis of extracts of various cell lines, using DNAJA2 antibody (CAB7059) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of C6 cells using DNAJA2 antibody (CAB7059) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using DNAJA2 antibody (CAB7059) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

DNAJA2 Antibody (PACO08871)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)