Anti-E2F6 Antibody (CAB11546)
- SKU:
- CAB11546
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-E2F6 Antibody |
| Antibody SKU: | CAB11546 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human E2F6 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | 293T, HepG2, RD |
| Immunogen: | A synthesized peptide derived from human E2F6 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 1876 |
| Uniprot: | O75461 |
| Cellular Location: | |
| Calculated MW: | 32kDa |
| Observed MW: | 36KDa |
 | Western blot analysis of extracts of various cell lines, using E2F6 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
 | Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug E2F6 antibody at a dilition of 1:1000. |
 | Chromatin immunoprecipitation analysis of extracts of 293T cells, using E2F6 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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