Anti-EGLN2 Antibody (CAB2252)

Antibody Name:	Anti-EGLN2 Antibody
Antibody SKU:	CAB2252
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EGLN2 (NP_444274.1).

Application:	WB IP
Recommended Dilution:	WB 1:500 - 1:2000 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, A-549, HepG2, U-87MG, MCF-7, Mouse brain, Rat heart

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EGLN2 (NP_444274.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSCS CSSG SGEA SAGL MEEA LPSA PERL ALDY IVPC MRYY GICV KDSF LGAA LGGR VLAE VEAL KRGG RLRD GQLV SQRA IPPR SIRG DQIA WVEG HEPG C
Gene ID:	112398
Uniprot:	Q96KS0
Cellular Location:	Nucleus
Calculated MW:	40kDa/43kDa
Observed MW:	43kDa

Synonyms:

EGLN2, EIT6, HIF-PH1, HIFPH1, HPH-1, HPH-3, PHD1

Background:

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.

UniProt Protein Function:	EGLN2: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. 2 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:	Protein type:EC 1.14.11.29; Oxidoreductase Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: nucleoplasm; cytosol; nucleus Molecular Function:protein binding; oxygen sensor activity; L-ascorbic acid binding; ferrous iron binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; peptidyl-proline 4-dioxygenase activity Biological Process: estrogen receptor signaling pathway; cell redox homeostasis; regulation of neuron apoptosis; positive regulation of protein catabolic process; response to hypoxia; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; regulation of cell growth
NCBI Summary:	The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]
UniProt Code:	Q96KS0
NCBI GenInfo Identifier:	32129513
NCBI Gene ID:	112398
NCBI Accession:	Q96KS0.1
UniProt Secondary Accession:	Q96KS0,Q8WWY4, Q9BV14, A8K5S0,
UniProt Related Accession:	Q96KS0
Molecular Weight:	407
NCBI Full Name:	Egl nine homolog 2
NCBI Synonym Full Names:	egl-9 family hypoxia-inducible factor 2
NCBI Official Symbol:	EGLN2
NCBI Official Synonym Symbols:	EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1
NCBI Protein Information:	egl nine homolog 2; estrogen-induced tag 6; HIF-prolyl hydroxylase 1; hypoxia-inducible factor prolyl hydroxylase 1; prolyl hydroxylase domain-containing protein 1
UniProt Protein Name:	Egl nine homolog 2
UniProt Synonym Protein Names:	Estrogen-induced tag 6; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; Prolyl hydroxylase domain-containing protein 1; PHD1
Protein Family:	Putative transcription factor
UniProt Gene Name:	EGLN2
UniProt Entry Name:	EGLN2_HUMAN

	Western blot analysis of extracts of various cell lines, using EGLN2 antibody (CAB2252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug EGLN2 antibody (CAB2252). Western blot was performed from the immunoprecipitate using EGLN2 antibody (CAB2252) at a dilition of 1:1000.

Antibodies

Anti-EGLN2 Antibody (CAB13447)

Anti-PHD1/prolyl hydroxylase Antibody (CAB3730)

EGLN2 Antibody (PACO09017)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)