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Cell Biology Antibodies 17 Anti-PHD1/prolyl hydroxylase Antibody CAB3730
Key Features

Prolyl hydroxylase PHD1 (EGLN2) Rabbit Monoclonal Antibody (CAB3730)

Datasheet MSDS
SKU CAB3730
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms EIT6, PHD1, EIT-6, HPH-1, HPH-3, HIFPH1, HIF-PH1, Prolyl hydroxylase PHD1 (EGLN2)
Research Use Cell Biology
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

Prolyl hydroxylase PHD1 (EGLN2) Monoclonal Antibody (CAB3730)

The Prolyl hydroxylase PHD1 (EGLN2) Monoclonal Antibody (CAB3730) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is optimized for detection of PHD1 protein in human samples and has been validated for use in Western blot applications.PHD1 is a key enzyme involved in the cellular response to low oxygen levels, known as hypoxia. It regulates the stability of hypoxia-inducible factors (HIFs), which control the expression of genes involved in processes such as angiogenesis, erythropoiesis, and metabolism.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Prolyl hydroxylase PHD1 (EGLN2) Monoclonal Antibody
SKU:CAB3730
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC0230
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MDSP CQPQ PLSQ ALPQ LPGS SSEP LEPE PGRA RMGV ESYL PCPL LPSY HCPG VPSE ASAG SGTP RATA TSTT ASPL RDGF GGQD GGEL RPLQ SEGA AALV
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:EIT6, PHD1, EIT-6, HPH-1, HPH-3, HIFPH1, HIF-PH1, Prolyl hydroxylase PHD1 (EGLN2)
Positive Sample:A-549, MCF7, PC-3, Mouse lung, Mouse testis, Mouse brain, Rat testis, Rat brain
Cellular Localization:Nucleus.
Calculated MW:44kDa
Observed MW:44kDa

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.

Purification MethodAffinity purification
Gene ID112398
RRIDAB_2863130
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb (CAB3730) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
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