The Prolyl hydroxylase PHD1 (EGLN2) Monoclonal Antibody (CAB3730) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is optimized for detection of PHD1 protein in human samples and has been validated for use in Western blot applications.PHD1 is a key enzyme involved in the cellular response to low oxygen levels, known as hypoxia. It regulates the stability of hypoxia-inducible factors (HIFs), which control the expression of genes involved in processes such as angiogenesis, erythropoiesis, and metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
A-549, MCF7, PC-3, Mouse lung, Mouse testis, Mouse brain, Rat testis, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
44kDa
Observed MW:
44kDa
The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
Purification Method
Affinity purification
Gene ID
112398
RRID
AB_2863130
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb (CAB3730) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
