Anti-Egl nine homolog 3 Antibody (CAB8001)
- SKU:
- CAB8001
- Product Type:
- Antibody
- Applications:
- WB
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | PHD3 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8001 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human PHD3 (NP_071356.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human PHD3 (NP_071356.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPLG HIMR LDLE KIAL EYIV PCLH EVGF CYLD NFLG EVVG DCVL ERVK QLHC TGAL RDGQ LAGP RAGV SKRH LRGD QITW IGGN EEGC EAIS FLLS LIDR LVLY CGSR LGKY YVKE RSKA |
| Gene ID: | 112399 |
| Uniprot: | Q9H6Z9 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 27kDa |
| Observed MW: | 27kDa |
| Synonyms: | EGLN3, HIFP4H3, HIFPH3, PHD3 |
| Background: |
| UniProt Protein Function: | EGLN3: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.14.11.29 Chromosomal Location of Human Ortholog: 14q13.1 Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol Molecular Function:protein binding; L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; peptidyl-proline 4-dioxygenase activity Biological Process: caspase activation; apoptosis; regulation of neuron apoptosis; response to hypoxia; protein amino acid hydroxylation; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; response to DNA damage stimulus; regulation of cell proliferation |
| UniProt Code: | Q9H6Z9 |
| NCBI GenInfo Identifier: | 32129515 |
| NCBI Gene ID: | 112399 |
| NCBI Accession: | Q9H6Z9.1 |
| UniProt Related Accession: | Q9H6Z9 |
| Molecular Weight: | Observed: 28 kDaPredicted: 28 kDa |
| NCBI Full Name: | Egl nine homolog 3 |
| NCBI Synonym Full Names: | egl-9 family hypoxia inducible factor 3 |
| NCBI Official Symbol: | EGLN3Â Â |
| NCBI Official Synonym Symbols: | PHD3; HIFPH3; HIFP4H3Â Â |
| NCBI Protein Information: | egl nine homolog 3 |
| UniProt Protein Name: | Egl nine homolog 3 |
| UniProt Synonym Protein Names: | HPH-1; Hypoxia-inducible factor prolyl hydroxylase 3; HIF-PH3; HIF-prolyl hydroxylase 3; HPH-3; Prolyl hydroxylase domain-containing protein 3; PHD3 |
| UniProt Gene Name: | EGLN3Â Â |
| UniProt Entry Name: | EGLN3_HUMAN |
 | Western blot analysis of extracts of mouse liver, using PHD3 antibody (CAB8001) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using PHD3 antibody (CAB8001) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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