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Anti-EIF2S3 Antibody (CAB3848)

SKU:
CAB3848
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
$167 - $503
Frequently bought together:

Description

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Antibody Name:Anti-EIF2S3 Antibody
Antibody SKU:CAB3848
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 173-472 of human EIF2S3 (NP_001406.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Rat
Positive Samples:Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 173-472 of human EIF2S3 (NP_001406.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HLAA IEIM KLKH ILIL QNKI DLVK ESQA KEQY EQIL AFVQ GTVA EGAP IIPI SAQL KYNI EVVC EYIV KKIP VPPR DFTS EPRL IVIR SFDV NKPG CEVD DLKG GVAG GSIL KGVL KVGQ EIEV RPGI VSKD SEGK LMCK PIFS KIVS LFAE HNDL QYAA PGGL IGVG TKID PTLC RADR MVGQ VLGA VGAL PEIF TELE ISYF LLRR LLGV RTEG DKKA AKVQ KLSK NEVL MVNI GSLS TGGR VSAV KADL GKIV LTNP VCTE VGEK IALS RRVE KHWR LIGW GQIR RGVT IKPT VDDD
Gene ID:1968
Uniprot:P41091
Cellular Location:
Calculated MW:51kDa
Observed MW:55kDa
Synonyms:EIF2S3, EIF2, EIF2G, EIF2gamma, MRXSBRK, eIF-2gA
Background:The protein encoded by this gene is the largest subunit of a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA(i) to the 40 S ribosomal subunit.
UniProt Protein Function:eIF2-gamma: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
UniProt Protein Details:

Protein type:Translation initiation; Translation

Chromosomal Location of Human Ortholog: Xp22.2-p22.1

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function:protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity

Biological Process: formation of translation preinitiation complex; translational initiation; transmembrane transport

NCBI Summary:The protein encoded by this gene is the largest subunit of a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA(i) to the 40 S ribosomal subunit. [provided by RefSeq, Jan 2010]
UniProt Code:P41091
NCBI GenInfo Identifier:729816
NCBI Gene ID:1968
NCBI Accession:P41091.3
UniProt Secondary Accession:P41091,Q53HK3, A0A024RBY4, A8K2Y2, B2R5N2, B5BTZ4,
UniProt Related Accession:P41091
Molecular Weight:51,109 Da
NCBI Full Name:Eukaryotic translation initiation factor 2 subunit 3
NCBI Synonym Full Names:eukaryotic translation initiation factor 2 subunit gamma
NCBI Official Symbol:EIF2S3  
NCBI Official Synonym Symbols:EIF2; EIF2G; eIF-2gA; EIF2gamma  
NCBI Protein Information:eukaryotic translation initiation factor 2 subunit 3
UniProt Protein Name:Eukaryotic translation initiation factor 2 subunit 3
UniProt Synonym Protein Names:Eukaryotic translation initiation factor 2 subunit gamma X; eIF-2-gamma X; eIF-2gX
Protein Family:Eukaryotic translation initiation factor
UniProt Gene Name:EIF2S3  
UniProt Entry Name:IF2G_HUMAN
Anti-EIF2S3 Antibody (CAB3848)Western blot analysis of extracts of rat brain, using EIF2S3 antibody (CAB3848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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