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Anti-EIF3E Antibody (CAB5447)

SKU:
CAB5447
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-EIF3E Antibody CAB5447
  • Metabolism Antibodies 2 Anti-EIF3E Antibody CAB5447
€139 - €419
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Description

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Antibody Name:Anti-EIF3E Antibody
Antibody SKU:CAB5447
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-445 of human EIF3E (NP_001559.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:MCF7, K-562, HepG2, Raji
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-445 of human EIF3E (NP_001559.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAEY DLTT RIAH FLDR HLVF PLLE FLSV KEIY NEKE LLQG KLDL LSDT NMVD FAMD VYKN LYSD DIPH ALRE KRTT VVAQ LKQL QAET EPIV KMFE DPET TRQM QSTR DGRM LFDY LADK HGFR QEYL DTLY RYAK FQYE CGNY SGAA EYLY FFRV LVPA TDRN ALSS LWGK LASE ILMQ NWDA AMED LTRL KETI DNNS VSSP LQSL QQRT WLIH WSLF VFFN HPKG RDNI IDLF LYQP QYLN AIQT MCPH ILRY LTTA VITN KDVR KRRQ VLKD LVKV IQQE SYTY KDPI TEFV ECLY VNFD FDGA QKKL RECE SVLV NDFF LVAC LEDF IENA RLFI FETF CRIH QCIS INML ADKL NMTP EEAE RWIV NLIR NARL DAKI DSKL GHVV MGNN AVSP YQQV IEKT KSLS FRSQ MLAM NIEK KLNQ NSRS EAPN WATQ DSGF Y
Gene ID:3646
Uniprot:P60228
Cellular Location:Cytoplasm, Nucleus, PML body
Calculated MW:52kDa
Observed MW:52kDa
Synonyms:EIF3E, EIF3-P48, EIF3S6, INT6, eIF3-p46
Background:
UniProt Protein Function:EIF3E: eukaryotic translation initiation factor 3 subunit 2. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits. Phosphorylated by TGF-beta type II receptor.
UniProt Protein Details:

Protein type:Translation initiation; Translation

Chromosomal Location of Human Ortholog: 8q22-q23

Cellular Component: eukaryotic translation initiation factor 3 complex; nucleoplasm; PML body; membrane; cytoplasm; cytosol; chromatin; nucleus

Molecular Function:protein binding; translation initiation factor activity; protein N-terminus binding

Biological Process: negative regulation of translational initiation; cellular protein metabolic process; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation

UniProt Code:P60228
NCBI GenInfo Identifier:41019126
NCBI Gene ID:3646
NCBI Accession:P60228.1
UniProt Secondary Accession:P60228,O43902, Q64058, Q64059, Q64252, Q6FG33, Q8WVK4
UniProt Related Accession:P60228
Molecular Weight:52,221 Da
NCBI Full Name:Eukaryotic translation initiation factor 3 subunit E
NCBI Synonym Full Names:eukaryotic translation initiation factor 3, subunit E
NCBI Official Symbol:EIF3E  
NCBI Official Synonym Symbols:INT6; EIF3S6; EIF3-P48; eIF3-p46  
NCBI Protein Information:eukaryotic translation initiation factor 3 subunit E; eIF-3 p48; mammary tumor-associated protein INT6; viral integration site protein INT-6 homolog; eukaryotic translation initiation factor 3 subunit 6; murine mammary tumor integration site 6 (oncogene homolog); eukaryotic translation initiation factor 3, subunit 6 48kDa
UniProt Protein Name:Eukaryotic translation initiation factor 3 subunit E
UniProt Synonym Protein Names:Eukaryotic translation initiation factor 3 subunit 6; Viral integration site protein INT-6 homolog; eIF-3 p48
UniProt Gene Name:EIF3E  
UniProt Entry Name:EIF3E_HUMAN
Anti-EIF3E Antibody (CAB5447)Western blot analysis of extracts of various cell lines, using EIF3E antibody (CAB5447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-EIF3E Antibody (CAB5447)Immunofluorescence analysis of HeLa cells using EIF3E antibody (CAB5447).

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