The ENTPD5 Monoclonal Antibody (CAB19642) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications including Western blot and immunofluorescence.ENTPD5, also known as ectonucleoside triphosphate diphosphohydrolase 5, is a multifunctional enzyme that plays a crucial role in regulating cellular nucleotide levels and maintaining cellular homeostasis. In particular, ENTPD5 is involved in the hydrolysis of extracellular nucleotides, which can impact various cellular processes such as cell proliferation, apoptosis, and immune responses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ENTPD5 Monoclonal Antibody
SKU:
CAB19642
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2210
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is similar to E-type nucleotidases (NTPases)/ecto-ATPase/apyrases. NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase-conserved regions which is characteristic of NTPases.
Purification Method
Affinity purification
Gene ID
957
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ENTPD5 Rabbit mAb (CAB19642) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from 293T cells, using ENTPD5 Rabbit mAb (CAB19642) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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