The F2RL3 Antibody (CAB8471) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in a variety of applications, including Western blotting. By binding specifically to the F2RL3 protein, this antibody allows for detailed detection and analysis in diverse cell types, making it an essential component for studies in the fields of immunology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
F2RL3 Antibody
SKU:
CAB8471
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PAR4, F2RL3
Positive Sample:
A-549, HT-29, Raji, B cells, Mouse spleen, Mouse lung, Mouse skeletal muscle, Rat spleen
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
41kDa
Observed MW:
41kDa
This gene encodes a member of the protease-activated receptor subfamily, part of the G-protein coupled receptor 1 family of proteins. The encoded receptor is proteolytically processed to reveal an extracellular N-terminal tethered ligand that binds to and activates the receptor. This receptor plays a role in blood coagulation, inflammation and response to pain. Hypomethylation at this gene may be associated with lung cancer in human patients.
Purification Method
Affinity purification
Gene ID
9002
RRID
AB_2769392
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using F2RL3 Rabbit pAb (CAB8471) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
