Anti-FBP1 Antibody (CAB5406)

Antibody Name:	Anti-FBP1 Antibody
Antibody SKU:	CAB5406
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-338 of human FBP1 (NP_000498.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-338 of human FBP1 (NP_000498.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADQ APFD TDVN TLTR FVME EGRK ARGT GELT QLLN SLCT AVKA ISSA VRKA GIAH LYGI AGST NVTG DQVK KLDV LSND LVMN MLKS SFAT CVLV SEED KHAI IVEP EKRG KYVV CFDP LDGS SNID CLVS VGTI FGIY RKKS TDEP SEKD ALQP GRNL VAAG YALY GSAT MLVL AMDC GVNC FMLD PAIG EFIL VDKD VKIK KKGK IYSL NEGY ARDF DPAV TEYI QRKK FPPD NSAP YGAR YVGS MVAD VHRT LVYG GIFL YPAN KKSP NGKL RLLY ECNP MAYV MEKA GGMA TTGK EAVL DVIP TDIH QRAP VILG SPDD VLEF LKVY EKHS AQ
Gene ID:	2203
Uniprot:	P09467
Cellular Location:
Calculated MW:	36kDa
Observed MW:	38KDa

Synonyms:	FBP1, FBP
Background:	Fructose-1, 6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis.

UniProt Protein Function:	FBPase: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans, FBP1 in the liver and FBP2 in muscle. While both forms are inhibited allosterically by AMP, NAD and Ca2+, the muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Deficiency of FBP1 leads to a disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
UniProt Protein Details:	Protein type:Phosphatase (non-protein); Carbohydrate Metabolism - pentose phosphate pathway; EC 3.1.3.11; Carbohydrate Metabolism - fructose and mannose; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis Chromosomal Location of Human Ortholog: 9q22.3 Cellular Component: cytoplasm; cytosol Molecular Function:monosaccharide binding; identical protein binding; protein binding; metal ion binding; fructose-bisphosphatase activity; AMP binding Biological Process: dephosphorylation; carbohydrate metabolic process; negative regulation of Ras protein signal transduction; glucose metabolic process; regulation of gluconeogenesis; pathogenesis; negative regulation of cell growth; negative regulation of glycolysis; fructose 6-phosphate metabolic process; protein homotetramerization; fructose metabolic process; gluconeogenesis Disease: Fructose-1,6-bisphosphatase Deficiency
NCBI Summary:	Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. [provided by RefSeq, Jul 2008]
UniProt Code:	P09467
NCBI GenInfo Identifier:	311033495
NCBI Gene ID:	2203
NCBI Accession:	P09467.5
UniProt Secondary Accession:	P09467,O75571, Q53F94, Q96E46,
UniProt Related Accession:	P09467
Molecular Weight:	338
NCBI Full Name:	Fructose-1,6-bisphosphatase 1
NCBI Synonym Full Names:	fructose-1,6-bisphosphatase 1
NCBI Official Symbol:	FBP1
NCBI Official Synonym Symbols:	FBP
NCBI Protein Information:	fructose-1,6-bisphosphatase 1; FBPase 1; liver FBPase; fructose-bisphosphatase 1; growth-inhibiting protein 17; D-fructose-1,6-bisphosphate 1-phosphohydrolase 1
UniProt Protein Name:	Fructose-1,6-bisphosphatase 1
UniProt Synonym Protein Names:	D-fructose-1,6-bisphosphate 1-phosphohydrolase 1; Liver FBPase
Protein Family:	Fat-body protein
UniProt Gene Name:	FBP1
UniProt Entry Name:	F16P1_HUMAN

	Western blot analysis of extracts of various cell lines, using FBP1 antibody (CAB5406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of C6 cells using FBP1 Rabbit pAb (CAB5406) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using FBP1 Rabbit pAb (CAB5406) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using FBP1 Rabbit pAb (CAB5406) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-FBP1 Antibody (CAB11664)	Human Fructose-1,6-bisphosphatase 1 (FBP1) ELISA Kit
FBP1 Antibody (PACO09244)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)