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Anti-FECH Antibody (CAB8418)

SKU:
CAB8418
Product Type:
Antibody
Applications:
WB
IHC
IF
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
  • Metabolism Antibodies 3 Anti-FECH Antibody CAB8418
  • Metabolism Antibodies 3 Anti-FECH Antibody CAB8418
  • Metabolism Antibodies 3 Anti-FECH Antibody CAB8418
  • Metabolism Antibodies 3 Anti-FECH Antibody CAB8418
  • Metabolism Antibodies 3 Anti-FECH Antibody CAB8418
€139 - €419
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Description

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Antibody Name:Anti-FECH Antibody
Antibody SKU:CAB8418
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human FECH (NP_000131.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, HT-29, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human FECH (NP_000131.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRSL GANM AAAL RAAG VLLR DPLA SSSW RVCQ PWRW KSGA AAAA VTTE TAQH AQGA KPQV QPQK RKPK TGIL MLNM GGPE TLGD VHDF LLRL FLDR DLMT LPIQ NKLA PFIA KRRT PKIQ EQYR RIGG GSPI KIWT SKQG EGMV KLLD ELSP NTAP HKYY IGFR YVHP LTEE AIEE MERD GLER AIAF TQYP QYSC STTG
Gene ID:2235
Uniprot:P22830
Cellular Location:Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:47kDa/48kDa
Observed MW:50kDa
Synonyms:FECH, EPP, FCE
Background:The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.
UniProt Code:P22830
NCBI GenInfo Identifier:60499021
NCBI Gene ID:2235
NCBI Accession:NP_000131
UniProt Secondary Accession:P22830,Q8IXN1, Q8NAN0, A8KA72,
UniProt Related Accession:P22830
Molecular Weight:48,625 Da
NCBI Full Name:ferrochelatase, mitochondrial isoform b
NCBI Synonym Full Names:ferrochelatase
NCBI Official Symbol:FECH  
NCBI Official Synonym Symbols:EPP; FCE  
NCBI Protein Information:ferrochelatase, mitochondrial; heme synthase; heme synthetase; protoheme ferro-lyase; protoporphyria
UniProt Protein Name:Ferrochelatase, mitochondrial
UniProt Synonym Protein Names:Heme synthase; Protoheme ferro-lyase
UniProt Gene Name:FECH  
UniProt Entry Name:HEMH_HUMAN
Anti-FECH Polyclonal Antibody (CAB8418)Western blot analysis of extracts of various cell lines, using FECH antibody (CAB8418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-FECH Polyclonal Antibody (CAB8418)Immunohistochemistry of paraffin-embedded human stomach using FECH antibody (CAB8418) at dilution of 1:100 (40x lens).
Anti-FECH Polyclonal Antibody (CAB8418)Immunohistochemistry of paraffin-embedded rat heart using FECH antibody (CAB8418) at dilution of 1:100 (40x lens).
Anti-FECH Polyclonal Antibody (CAB8418)Immunohistochemistry of paraffin-embedded mouse lung using FECH antibody (CAB8418) at dilution of 1:100 (40x lens).
Anti-FECH Polyclonal Antibody (CAB8418)Immunohistochemistry of paraffin-embedded mouse stomach using FECH antibody (CAB8418) at dilution of 1:100 (40x lens).

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