The Flag-Tag (DYKDDDDK Tag) Protein ELISA Kit is a reliable and sensitive assay designed for the quantitative measurement of Flag-tagged proteins in various biological samples. This kit offers high specificity and accuracy, providing researchers with consistent and reproducible results for a wide range of applications. The Flag-Tag (DYKDDDDK Tag) is a commonly used epitope tag that allows for easy detection and purification of recombinant proteins. By using this ELISA kit, researchers can confidently assess the expression levels of Flag-tagged proteins in cell lysates, culture supernatants, or serum samples.
Whether studying protein expression, protein-protein interactions, or protein localization, the Flag-Tag (DYKDDDDK Tag) Protein ELISA Kit is a valuable tool for researchers in the fields of molecular biology, biochemistry, and biotechnology. Trust in this kit for precise and accurate quantification of Flag-tagged proteins in your experimental samples.
Product Name:
FLAG-Tag (DYKDDDDK-Tag) ELISA Kit
SKU:
AEFI01016
Reactivity:
Universal
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
9.375 ng/mL
Range:
15.625-1000 ng/mL
Sample Type:
Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Cell Culture Lysate (n = 5)
82-98%
84-101%
90-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Cell Culture Lysate (n = 5)
85-105
95
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
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