Anti-DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG® M2 Antibody)

DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG® M2 Antibody)

DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG® M2 Antibody) is a rabbit polyclonal antibody directed against DYKDDDDK Tag (Equivalent to Sigma's Anti-FLAG® M2), offered by Assay Genie for research applications. Reported applications include WB. Reactivity is species independent. Purified by Peptide Affinity.

The DYKDDDDK tag, also known as the FLAG® tag, is a widely used epitope tag in molecular biology and protein research. Comprising eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), this hydrophilic sequence is engineered into recombinant proteins to facilitate detection, purification, and localization studies. Its small size minimizes interference with protein function, making it ideal for fusion protein applications in both prokaryotic and eukaryotic systems. Anti-DYKDDDDK tag antibodies, such as those equivalent to Sigma’s Anti-FLAG® M2, are monoclonal or polyclonal antibodies specifically designed to recognize the DYKDDDDK epitope with high affinity and specificity. These antibodies are essential tools in Western blotting, immunoprecipitation, immunofluorescence, and ELISA, enabling researchers to track tagged proteins with precision. The FLAG tag system is particularly valued for its compatibility with gentle elution conditions, preserving protein structure and function during purification. Additionally, the tag’s unique sequence ensures minimal cross-reactivity with endogenous proteins, enhancing signal clarity in complex biological samples. Anti-DYKDDDDK antibodies are validated across multiple species and applications, making them a cornerstone in proteomics, structural biology, and therapeutic protein development. Their robust performance and commercial availability have established them as a gold standard in epitope tagging technologies. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.