Formate Assay Kit - Colorimetric (MAES0095)
- SKU:
- MAES0095
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Animal Tissue
- Assay Time:
- 70 min
- Sensitivity:
- 8.20 umol/L
- Range:
- 8.20-800 umol/L
- Shipping:
- Gel Pack
- Detection Method:
- Colorimetric
- Research Area:
- Metabolism
Description
| Product Name: | Formate Assay Kit - Colorimetric |
| Product Code: | MAES0095 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, animal tissue |
| Assay Time: | 70 min |
| Instrument: | Microplate reader |
| Sensitivity: | 8.20 µmol/L |
| Detection Range: | 8.20-800 µmol/L |
| Recovery Rate: | 100 |
| Inter CV: | 3.9 |
| Inter CV: | 3.3 |
Formic acid is the simplest carboxylic acid, whose chemical formula is CH2O2. It is often used as an antimicrobial/preservative in livestock feed. Formic acid can block some of the decay processes in feed, making the nutritional value of feed maintain longer. Under normal circumstances, the physiological concentration of formic acid is low and easy to metabolize, but in the case of methanol poisoning, the concentration can reach 5 mmoL/L. Similarly, long-term exposure to excessive levels of formaldehyde can also increase the content of formic acid in blood and urine.
Formic acid dehydrogenase (FDH) catalyzes the reaction of formic acid with NAD+ to produce NADH. NADH, under the action of PMS, transfers electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. The NAD(P)H in the sample itself will cause certain background interference, so set the control well in the measurement process to eliminate such interference.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
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