Anti-GABPB1 Antibody (CAB6909)

Antibody Name:	Anti-GABPB1 Antibody
Antibody SKU:	CAB6909
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 231-395 of human GABPB1 (NP_005245.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	MCF7, HepG2, BT-474, HT-29, Mouse spleen, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 231-395 of human GABPB1 (NP_005245.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AEAS APLS NSSE TPVV ATEE VVTA ESVD GAIQ QVVS SGGQ QVIT IVTD GIQL GNLH SIPT SGIG QPII VTMP DGQQ VLTV PATD IAEE TVIS EEPP AKRQ CIEI IENR VESA EIEE REAL QKQL DEAN REAQ KYRQ QLLK KEQE AEAY RQKL EAMT RLQT NKEA V
Gene ID:	2553
Uniprot:	Q06547
Cellular Location:	Nucleus
Calculated MW:	36kDa/38kDa/41kDa/42kDa
Observed MW:	50kDa

Synonyms:

GABPB1, BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB-1, GABPB2, NRF2B1, NRF2B2

Background:

This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

UniProt Protein Function:	GABPB1: Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 15q21.2 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; protein heterodimerization activity; transcription factor activity Biological Process: regulation of transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; transcription, DNA-dependent; organelle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q06547
NCBI GenInfo Identifier:	23503070
NCBI Gene ID:	2553
NCBI Accession:	Q06547.2
UniProt Secondary Accession:	Q06547,Q06545, Q12940, Q12941, Q12942, Q8IYD0, A8IE52
UniProt Related Accession:	Q06547
Molecular Weight:	395
NCBI Full Name:	GA-binding protein subunit beta-1
NCBI Synonym Full Names:	GA binding protein transcription factor, beta subunit 1
NCBI Official Symbol:	GABPB1
NCBI Official Synonym Symbols:	E4TF1; GABPB; BABPB2; E4TF1B; GABPB2; NRF2B1; NRF2B2; E4TF1-47; E4TF1-53
NCBI Protein Information:	GA-binding protein subunit beta-1; GABPB-1; GABP subunit beta-2; nuclear respiratory factor 2; transcription factor E4TF1-47; transcription factor E4TF1-53; GA binding protein transcription factor beta subunit 1 transcript variant gamma-2
UniProt Protein Name:	GA-binding protein subunit beta-1
UniProt Synonym Protein Names:	GABP subunit beta-2; GABPB-2; Nuclear respiratory factor 2; Transcription factor E4TF1-47; Transcription factor E4TF1-53
Protein Family:	GA-binding protein
UniProt Gene Name:	GABPB1
UniProt Entry Name:	GABP1_HUMAN

	Western blot analysis of extracts of various cell lines, using GABPB1 antibody (CAB6909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse kidney using GABPB1 Antibody (CAB6909) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using GABPB1 Antibody (CAB6909) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using GABPB1 Antibody (CAB6909) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using GABPB1 antibody (CAB6909).

Antibodies

GABPB1 Antibody (PACO09382)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)