Anti-GDAP1 Antibody (CAB6601)

Antibody Name:	Anti-GDAP1 Antibody
Antibody SKU:	CAB6601
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human GDAP1 (NP_001035808.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HepG2, Mouse brain, Mouse spinal cord, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human GDAP1 (NP_001035808.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRLN STGE VPVL IHGE NIIC EATQ IIDY LEQT FLDE RTPR LMPD KESM YYPR VQHY RELL DSLP MDAY THGC ILHP ELTV DSMI PAYA TTRI RSQI GNTE SELK KLAE ENPD LQEA YIAK QKRL KSKL LDHD NVKY LKKI LDEL EKVL DQVE TELQ RRNE ETPE EGQQ PWLC GESF TLAD VSLA VTLH RLKF LGFA RRNW GNGK RPNL ETYY ERVL KRKT FNKV L
Gene ID:	54332
Uniprot:	Q8TB36
Cellular Location:	Cytoplasm, Mitochondrion outer membrane, Multi-pass membrane protein
Calculated MW:	33kDa/41kDa
Observed MW:	38kDa

Synonyms:

GDAP1, CMT4, CMT4A, CMTRIA

Background:

This gene encodes a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy. Two transcript variants encoding different isoforms and a noncoding variant have been identified for this gene.

UniProt Protein Function:	GDAP1: the ganglioside-induced differentiation associated protein-1 gene causes autosomal recessive demyelinating or axonal Charcot-Marie-Tooth neuropathy (CMT).
UniProt Protein Details:	Protein type:Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 8q21.11 Cellular Component: mitochondrion; membrane; cytoplasm; nucleus; integral to mitochondrial outer membrane Biological Process: mitochondrial fission; response to retinoic acid; protein targeting to mitochondrion Disease: Charcot-marie-tooth Disease, Axonal, With Vocal Cord Paresis, Autosomal Recessive; Charcot-marie-tooth Disease, Axonal, Type 2k; Charcot-marie-tooth Disease, Type 4a; Charcot-marie-tooth Disease, Recessive Intermediate A
NCBI Summary:	This gene encodes a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy. Two transcript variants encoding different isoforms and a noncoding variant have been identified for this gene. [provided by RefSeq, Feb 2012]
UniProt Code:	Q8TB36
NCBI GenInfo Identifier:	269849682
NCBI Gene ID:	54332
NCBI Accession:	Q8TB36.3
UniProt Secondary Accession:	Q8TB36,A8K957, E7FJF3, E7FJF4,
UniProt Related Accession:	Q8TB36
Molecular Weight:	358
NCBI Full Name:	Ganglioside-induced differentiation-associated protein 1
NCBI Synonym Full Names:	ganglioside induced differentiation associated protein 1
NCBI Official Symbol:	GDAP1
NCBI Official Synonym Symbols:	CMT4; CMT4A; CMTRIA
NCBI Protein Information:	ganglioside-induced differentiation-associated protein 1; Charcot-Marie-Tooth neuropathy 4A; ganglioside differentiation associated protein 1
UniProt Protein Name:	Ganglioside-induced differentiation-associated protein 1
Protein Family:	Ganglioside-induced differentiation-associated protein
UniProt Gene Name:	GDAP1
UniProt Entry Name:	GDAP1_HUMAN

	Western blot analysis of extracts of various cell lines, using GDAP1 antibody (CAB6601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using GDAP1 antibody (CAB6601) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using GDAP1 antibody (CAB6601) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using GDAP1 antibody (CAB6601) at dilution of 1:100 (40x lens).

Antibodies

GDAP1 Antibody (PACO09439)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)