The General Prostacyclin (PGI2) ELISA Kit is a highly reliable and accurate tool for the detection of prostacyclin levels in various biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and reproducible results for a wide range of research applications.Prostacyclin, also known as PGI2, is a key prostaglandin involved in numerous physiological processes, including vasodilation, platelet aggregation inhibition, and regulation of inflammation. Dysregulation of prostacyclin levels has been implicated in various diseases such as cardiovascular disorders, pulmonary hypertension, and inflammatory conditions, highlighting its importance as a potential biomarker for disease monitoring and therapeutic development.
With the General Prostacyclin (PGI2) ELISA Kit, researchers can accurately quantify prostacyclin levels in biological samples, facilitating a deeper understanding of its role in health and disease. This kit is a valuable tool for studying prostacyclin-related pathways and developing novel strategies for disease diagnosis and treatment.
Product Name:
General Prostacyclin (PGI2) ELISA Kit
Product Code:
UNDL00066
Size:
96 Assays
Synonyms:
prostaglandin I2
Detection Method:
Competition
Range:
78.1-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
