The PGI2 (Prostacyclin) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of prostacyclin levels in biological samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an invaluable tool for researchers in various fields.Prostacyclin, also known as PGI2, is a key mediator in inflammation and vascular health. It plays a crucial role in maintaining cardiovascular homeostasis and regulating blood flow, making it a promising therapeutic target for cardiovascular diseases. By accurately measuring prostacyclin levels, researchers can gain insights into the mechanisms underlying these conditions and develop targeted treatment strategies.In conclusion, the PGI2 (Prostacyclin) ELISA Kit is a valuable resource for researchers seeking to investigate the role of prostacyclin in various biological processes. Its high sensitivity and specificity ensure precise and reliable results, making it an indispensable tool for studying cardiovascular health and developing innovative therapeutic interventions.
Product Name:
PGI2 (Prostacyclin) ELISA Kit
SKU:
UNES00016
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
