The General Vitamin A (VA) ELISA Kit from Assay Genie is a cutting-edge tool designed for the accurate measurement of Vitamin A levels in various biological samples including serum, plasma, and tissue lysates. This kit boasts high sensitivity and specificity, providing researchers with reliable and reproducible results for a wide range of applications.Vitamin A plays a crucial role in maintaining healthy vision, immune function, and cell growth and differentiation. Deficiencies in Vitamin A have been linked to a variety of health conditions such as night blindness, dry skin, and impaired immune response.
By accurately measuring and monitoring Vitamin A levels, researchers can gain valuable insights into nutritional status and potential health implications.With the General Vitamin A (VA) ELISA Kit, researchers can confidently study the impact of Vitamin A on human health, evaluate dietary intake, and assess the effectiveness of supplementation strategies. This kit is a valuable tool for researchers in the fields of nutrition, public health, and clinical research, providing essential data for advancing our understanding of Vitamin A and its role in overall wellness.
Product Name:
General Vitamin A (VA) ELISA Kit
Product Code:
UNDL00077
Size:
96 Assays
Target:
VA
Synonyms:
Vitamin A, Aquasola, Ret
Detection Method:
Competition
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
