The Vitamin A ELISA Kit is designed for the quantitative detection of Vitamin A levels in various biological samples. Vitamin A is essential for numerous biological mechanisms, including vision, immunity, growth, and cellular differentiation. It plays a critical role in maintaining the health of visual system and epithelial cells. Under or over levels of Vitamin A has been implicated in several diseases such as night blindness, hyperkeratosis, and toxicity. Accurate measurement of Vitamin A is vital for understanding its role in these disease mechanisms and for developing appropriate therapeutic strategies. Assay Genie's Vitamin A ELISA Kit provides exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit ensures robust performance and is user-friendly, making it an ideal choice for both research and clinical applications. Trust Assay Genie's Vitamin A ELISA Kit for accurate and dependable quantification of this vital biomarker in your studies.
Product Name:
VA (Vitamin A) ELISA Kit
SKU:
AEES00010
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
