The GenieFluor 488 Anti-Human CD28 Antibody (CD28-2) available at www.assaygenie.com is a cutting-edge tool for researchers studying CD28, a crucial co-stimulatory molecule involved in immune cell activation. This antibody, optimized for use in a variety of applications, is specifically designed to target human CD28 with high sensitivity and specificity. Researchers can utilize this antibody in flow cytometry, immunofluorescence, and immunohistochemistry experiments to detect and analyze CD28 expression in different cell types.CD28 is a pivotal receptor found on T cells that provides a vital secondary signal for their activation and proliferation. By engaging with its ligands, CD28 plays a central role in shaping the adaptive immune response and is instrumental in controlling T cell activation thresholds.
Understanding the function and regulation of CD28 is essential for investigating immune responses, tolerance, and immunotherapies in diseases such as cancer, autoimmune disorders, and infectious diseases.The GenieFluor 488 Anti-Human CD28 Antibody (CD28-2) offers researchers a powerful tool to delve deeper into the complex mechanisms of immune activation and regulation. Its versatility and reliability make it a valuable asset for studies aiming to unravel the intricacies of CD28 signaling pathways and their implications in various disease settings.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P10747
Gene ID:
940
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
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