GeniePlex Human Inflammatory Chemokine 7-Plex Panel 1 (96 Tests)

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ELISA Kit Technical Manual

Product Name: GeniePlex Human Inflammatory Chemokine 7-Plex Immunoassay Panel 1


BCA-1, MCP-1, MIP-1-Alpha, MIP-3-Beta, PARC, PF4, RANTES

Species Reactivity:


Assay Type:


Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex GeniePlex Human Inflammatory Chemokine 7-Plex Immunoassay Panel 1 is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes BCA-1, MCP-1, MIP-1-Alpha, MIP-3-Beta, PARC, PF4, RANTES on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 7 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Analytes:BCA-1, MCP-1, MIP-1-Alpha, MIP-3-Beta, PARC, PF4, RANTES
1x Ab-conjugated beads (premixed):S4P2 - anti-H RANTES; S4P9 - anti-H MIP-1-Alpha; S4P11 - anti-H MCP-1; S5P2 - anti-H BCA-1; S5P4 - anti-H PF4; S5P8 - anti-H MIP-3-Beta; S5P9 - anti-H PARC
2x Biotin-detection Ab (premixed):Biotin - anti-H RANTES; Biotin - anti-H MIP-1-Alpha; Biotin - anti-H MCP-1; Biotin - anti-H BCA-1; Biotin - anti-H PF4; Biotin - anti-H MIP-3-Beta; Biotin - anti-H PARC
Buffers:2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix:Lyophilized Standard Mix-Human Inflammatory Chemokine 7-Plex Panel 1. One vial containing lyophilized recombinant BCA-1, MCP-1, MIP-1-Alpha, MIP-3 Beta, PARC, PF4, RANTES.
Sample types:Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs):BCA-1, < 10 pg/mL; MCP-1, < 2 pg/mL; MIP-1-Alpha, < 2 pg/mL; MIP-3-Beta, < 10 pg/mL; PARC, < 5 pg/mL; PF4, < 5 pg/mL; RANTES, < 5 pg/mL.
LLOQs:BCA-1, < 20 pg/mL; MCP-1, < 5 pg/mL; MIP-1-Alpha, < 5 pg/mL; MIP-3-Beta, < 20 pg/mL; PARC, < 10 pg/mL; PF4, < 10 pg/mL; RANTES, < 10 pg/mL.
ULOQs:BCA-1,  > 5,000 pg/mL; MCP-1,  > 5,000 pg/mL; MIP-1-Alpha,  > 2,000 pg/mL; MIP-3-Beta,  > 5,000 pg/mL; PARC,  > 2,000 pg/mL; PF4,  > 2,000 pg/mL; RANTES,  > 5,000 pg/mL.
Standard dose recovery:Standard dose recovery: 70-130%
Intra-assay CV:Intra-assay CV: < 10%
Inter-assay CV:Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Cross-reactivity of analytes in the Panel: Negligible
Sample volume:Sample volume: 15 µL/test


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