GeniePlex Human Chemokine 6-Plex (96 Tests)

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ELISA Kit Technical Manual

Product Name: GeniePlex Human Chemokine 6-Plex Immunoassay Panel


Eotaxin, IL-8, RANTES, MCP-1, MCP-3, IP-10

Species Reactivity:


Assay Type:


Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex GeniePlex Human Chemokine 6-Plex Immunoassay Panel is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes Eotaxin, IL-8, RANTES, MCP-1, MCP-3, IP-10 on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 6 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Analytes:Eotaxin, IL-8, RANTES, MCP-1, MCP-3, IP-10
1x Ab-conjugated beads (premixed):S4P1 - anti-human Eotaxin; S4P4 - anti-human IP-10; S4P10 - anti-human IL-8; S5P1 - anti-human MCP-1; S5P3 - anti-human MCP-3; S5P7 - anti-human RANTES
2x Biotin-detection Ab (premixed):Biotin - anti-human Eotaxin; Biotin - anti-human IP-10; Biotin - anti-human IL-8; Biotin - anti-human MCP-1; Biotin - anti-human MCP-3; Biotin - anti-human RANTES
Buffers:2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix:Lyophilized Standard Mix-Human Chemokine 6-Plex. One vial containing lyophilized recombinant Eotaxin, IL-8, IP-10, MCP-1, MCP-3, RANTES.
Sample types:Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs):Eotaxin, < 5 pg/mL; IL-8, < 1 pg/mL; IP-10, < 3 pg/mL; MCP-1, < 2 pg/mL; MCP-3, < 2 pg/mL; RANTES, < 5 pg/mL.
LLOQs:Eotaxin, < 10 pg/mL; IL-8, < 1 pg/mL; IP-10, < 10 pg/mL; MCP-1, < 5 pg/mL; MCP-3, < 5 pg/mL; RANTES, < 10 pg/mL.
ULOQs:Eotaxin, > 2,000 pg/mL; IL-8, > 1,000 pg/mL; IP-10, > 2,000 pg/mL; MCP-1, > 5,000 pg/mL; MCP-3,  > 5,000 pg/mL; RANTES,  > 5,000 pg/mL..
Standard dose recovery:Standard dose recovery: 70-130%
Intra-assay CV:Intra-assay CV: < 10%
Inter-assay CV:Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Cross-reactivity of analytes in the Panel: Negligible
Sample volume:Sample volume: 15 µL/test


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