Description
Product Name: GeniePlex Human Th1/Th2/Th17 12-plex
Analytes
IFN-Gamma, IL-1-Beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, TNF-Alpha, TNF-BetaSpecies Reactivity:
Human
Assay Type:
Multiplex
Detection Method:
Flow Cytometry
Assay Time:
2 hours
Product Overview
This GeniePlex GeniePlex Human Th1/Th2/Th17 12-plex is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IFN-Gamma, IL-1-Beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, TNF-Alpha, TNF-Beta on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 12 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.
As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.
Product Information
Reactivity: | Human |
Analytes: | IFN-Gamma, IL-1-Beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, TNF-Alpha, TNF-Beta |
1x Ab-conjugated beads (premixed): | S4P2 - anti-human TNF-Beta; S4P3 - anti-human TNF-Alpha; S4P6 - anti-human IL-1-Beta; S4P7 - anti-human IL-2; S4P9 - anti-human IFN-Gamma; S4P10 - anti-human IL-8; S4P12 - anti-human IL-12p70; S5P4 - anti-human IL-4; S5P5 - anti-human IL-5; S5P6 - anti-human IL-6; S5P9 - anti-human IL-17A; S5P10 - anti-human IL-10 |
2x Biotin-detection Ab (premixed): | Biotin - anti-human TNF-Beta; Biotin - anti-human TNF-Alpha; Biotin - anti-human IL-1-Beta; Biotin - anti-human IL-2; Biotin - anti -human IFN-Gamma; Biotin - anti-human IL-8; Biotin - anti-human IL-12p70; Biotin - anti-human IL-4; Biotin - anti-human IL-5; Biotin - anti-human IL-6; Biotin - anti-human IL-17A; Biotin - anti-human IL-10 |
Buffers: | 2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent. |
Lyophilized Standard Mix: | Lyophilized Standard Mix- Human TH1/Th2/Th17 12-plex. Vial A and Vial B containing lyophilized recombinant human IFN-Gamma, IL-1 Beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, TNF-Alpha, TNF Beta. |
Sample types: | Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate |
Sensitivity (LODs): | IFN-Gamma, < 3 pg/mL; IL-1-Beta, < 5 pg/mL; IL-2, < 3 pg/mL; IL-4, < 1 pg/mL; IL-5, < 2 pg/mL; IL-6, < 5 pg/mL; IL-8, < 1 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 3 pg/mL; IL-17A, < 3 pg/mL; TNF-Alpha, < 1 pg/mL; TNF-Beta, < 5 pg/mL. |
LLOQs: | IFN-Gamma, < 10 pg/mL; IL-1-Beta, < 10 pg/mL; IL-2, < 10 pg/mL; IL-4, < 3 pg/mL; IL-5, < 5 pg/mL; IL-6, < 10 pg/mL; IL-8, < 1 pg/mL; IL-10, < 5 pg/mL; IL-12p70, < 10 pg/mL; IL-17A, < 10 pg/mL; TNF-Alpha, < 2 pg/mL; TNF-Beta, < 10 pg/mL. |
ULOQs: | IFN-Gamma, > 2,000 pg/mL; IL-1-Beta, > 5,000 pg/mL; IL-2, > 2,000 pg/mL; IL-4, > 1,000 pg/mL; IL-5, > 2,000 pg/mL; IL-6, > 5,000 pg/mL; IL-8, > 1,000 pg/mL; IL-10, > 5,000 pg/mL; IL-12p70, > 2,000 pg/mL; IL-17A, > 1,000 pg/mL; TNF-Alpha, > 1,000 pg/mL; TNF-Beta, > 5,000 pg/mL. |
Standard dose recovery: | Standard dose recovery: 70-130% |
Intra-assay CV: | Intra-assay CV: < 10% |
Inter-assay CV: | Inter-assay CV: < 20% |
Cross-reactivity of analytes in the Panel: | Cross-reactivity of analytes in the Panel: Negligible |
Sample volume: | Sample volume: 15 µL/test |
Additional Information
Product type: |
Multiplex |
Reactivity: |
Human |
ELISA Type: |
Multiplex |