Description
GenieScript Reverse Transcriptase
GenieScript Reverse Transcriptase is a high-activity RNA-dependent DNA polymerase for first-strand cDNA synthesis from RNA templates. It supports robust reverse transcription across a broad range of template amounts and is well suited to downstream PCR, qPCR and library-preparation workflows.
The enzyme is supplied with an optimised reaction buffer and can be primed with oligo(dT), random primers or gene-specific primers, giving flexibility for both targeted and whole-transcriptome applications.
| Product Name: | GenieScript Reverse Transcriptase |
| Brand: | GenieScript |
| Product type: | Reverse transcriptase (RNA-dependent DNA polymerase) |
| Priming options: | Oligo(dT), random primers, or gene-specific primers |
| Supplied with: | Optimised first-strand reaction buffer |
| Storage: | Store at -20 C. Keep on ice during use. |
| Shipping: | Ships on dry ice / blue ice. |
| Available sizes: | 10,000 U, 40,000 U |
- High cDNA yield across a wide range of input RNA amounts.
- Compatible with oligo(dT), random and gene-specific priming.
- Supplied with an optimised first-strand reaction buffer.
- Reliable performance feeding into RT-PCR, RT-qPCR and cDNA library construction.
- First-strand cDNA synthesis
- Two-step RT-PCR and RT-qPCR
- cDNA library construction
- Gene-expression analysis
| Step | Procedure |
| 1 | Combine template RNA with your chosen primer and dNTPs; denature briefly and cool on ice. |
| 2 | Add the reaction buffer and GenieScript Reverse Transcriptase to assemble the first-strand reaction. |
| 3 | Incubate for cDNA synthesis at the recommended temperature. |
| 4 | Heat-inactivate the enzyme, then use the cDNA directly in downstream PCR/qPCR. |
Note: Reaction temperatures, times and unit amounts should be optimised for your template; refer to the product insert for detailed set-up guidance.
What can I prime the reaction with?
GenieScript works with oligo(dT) for polyadenylated mRNA, random primers for total RNA, or gene-specific primers for targeted cDNA synthesis.
What are the available pack sizes?
It is offered in 10,000 U and 40,000 U pack sizes to suit both routine and higher-throughput use.
How should the enzyme be stored?
Store at -20 C and keep it on ice while setting up reactions to preserve activity.
