Anti-GFRA1 Antibody (CAB5373)

Antibody Name:	Anti-GFRA1 Antibody
Antibody SKU:	CAB5373
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human GFRA1 (NP_665736.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human GFRA1 (NP_665736.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DRLD CVKA SDQC LKEQ SCST KYRT LRQC VAGK ETNF SLAS GLEA KDEC RSAM EALK QKSL YNCR CKRG MKKE KNCL RIYW SMYQ SLQG NDLL EDSP YEPV NSRL SDIF RVVP FISV EHIP KGNN CLDA AKAC NLDD ICKK YRSA YITP CTTS VSND VCNR RKCH KALR QFFD KVPA KHSY GMLF CSCR DIAC TERR RQTI VPVC SYEE REKP NCLN LQDS CKTN YICR SRLA DFFT NCQP ESRS VSSC LKEN YADC
Gene ID:	2674
Uniprot:	P56159
Cellular Location:	Cell membrane, GPI-anchor, Lipid-anchor
Calculated MW:	50kDa/51kDa
Observed MW:	60kDa

Synonyms:

GFRA1, GDNFR, GDNFRA, GFR-ALPHA-1, RET1L, RETL1, TRNR1

Background:

This gene encodes a member of the glial cell line-derived neurotrophic factor receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature receptor. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. This receptor is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

UniProt Protein Function:	GFRA1: Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, GPI anchor; Receptor, misc. Chromosomal Location of Human Ortholog: 10q26.11 Cellular Component: extrinsic to membrane; plasma membrane Molecular Function:glial cell line-derived neurotrophic factor receptor activity; receptor binding Biological Process: axon guidance; cell surface receptor linked signal transduction
NCBI Summary:	Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Feb 2009]
UniProt Code:	P56159
NCBI GenInfo Identifier:	20141405
NCBI Gene ID:	2674
NCBI Accession:	P56159.2
UniProt Secondary Accession:	P56159,O15507, O43912, A8KA21,
UniProt Related Accession:	P56159
Molecular Weight:
NCBI Full Name:	GDNF family receptor alpha-1
NCBI Synonym Full Names:	GDNF family receptor alpha 1
NCBI Official Symbol:	GFRA1
NCBI Official Synonym Symbols:	GDNFR; RET1L; RETL1; TRNR1; GDNFRA; GFR-ALPHA-1
NCBI Protein Information:	GDNF family receptor alpha-1; RET ligand 1; GDNFR-alpha-1; GDNF receptor alpha-1; GPI-linked anchor protein; PI-linked cell-surface accessory protein; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; Glial cell line-derived neurotrophic factor receptor alpha
UniProt Protein Name:	GDNF family receptor alpha-1
UniProt Synonym Protein Names:	RET ligand 1; TGF-beta-related neurotrophic factor receptor 1
Protein Family:	GDNF family receptor
UniProt Gene Name:	GFRA1
UniProt Entry Name:	GFRA1_HUMAN

	Western blot analysis of extracts of various cell lines, using GFRA1 antibody (CAB5373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human thyroid cancer using GFRA1 Rabbit pAb (CAB5373) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using GFRA1 Rabbit pAb (CAB5373) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using GFRA1 Rabbit pAb (CAB5373) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using GFRA1 Rabbit pAb (CAB5373) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-GFRA1 Antibody (CAB2953)	Human GFRA1 / GDNF Receptor alpha 1 ELISA Kit
GFRA1 Antibody (PACO03858)	Human GDNF family receptor alpha-1 (GFRA1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)