Anti-GGA2 Antibody (CAB1186)

Antibody Name:	Anti-GGA2 Antibody
Antibody SKU:	CAB1186
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GGA2 (NP_055859.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HepG2, Raji, MCF7, THP-1, 22Rv1, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GGA2 (NP_055859.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAT AVAA AVAG TESA QGPP GPAA SLEL WLNK ATDP SMSE QDWS AIQN FCEQ VNTD PNGP THAP WLLA HKIQ SPQE KEAL YALT VLEM CMNH CGEK FHSE VAKF RFLN ELIK VLSP KYLG SWAT GKVK GRVI EILF SWTV WFPE DIKI RDAY QMLK KQGI IKQD PKLP VDKI LPPP SPWP KSSI FDAD EEKS KLLT RLLK SNHP EDLQ AANR LIKN LVKE EQEK SEKV SKRV SAVE EVRS HVKV LQEM LSMY RRPG QAPP DQEA LQVV YERC EKLR PTLF RLAS DTTD DDDA LAEI LQAN
Gene ID:	23062
Uniprot:	Q9UJY4
Cellular Location:	Endosome membrane, Golgi apparatus, Peripheral membrane protein, trans-Golgi network membrane
Calculated MW:	67kDa
Observed MW:	72-80kDa

Synonyms:

GGA2, VEAR

Background:

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly.

UniProt Protein Function:	GGA2: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Belongs to the GGA protein family.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Vesicle Chromosomal Location of Human Ortholog: 16p12 Cellular Component: Golgi apparatus; clathrin adaptor complex; clathrin-coated vesicle; endosome membrane; trans-Golgi network Molecular Function:ADP-ribosylation factor binding; protein binding Biological Process: vesicle-mediated transport; intracellular protein transport
NCBI Summary:	This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UJY4
NCBI GenInfo Identifier:	143811397
NCBI Gene ID:	23062
NCBI Accession:	Q9UJY4.3
UniProt Related Accession:	Q9UJY4
Molecular Weight:	~ 72-80kDa
NCBI Full Name:	ADP-ribosylation factor-binding protein GGA2
NCBI Synonym Full Names:	golgi associated, gamma adaptin ear containing, ARF binding protein 2
NCBI Official Symbol:	GGA2
NCBI Official Synonym Symbols:	VEAR
NCBI Protein Information:	ADP-ribosylation factor-binding protein GGA2
UniProt Protein Name:	ADP-ribosylation factor-binding protein GGA2
UniProt Synonym Protein Names:	Gamma-adaptin-related protein 2; Golgi-localized, gamma ear-containing, ARF-binding protein 2; VHS domain and ear domain of gamma-adaptin; Vear
Protein Family:	ADP-ribosylation factor-binding protein
UniProt Gene Name:	GGA2
UniProt Entry Name:	GGA2_HUMAN

	Western blot analysis of extracts of various cell lines, using GGA2 antibody (CAB1186) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human kidney using GGA2 Antibody (CAB1186) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using GGA2 Antibody (CAB1186) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using GGA2 Antibody (CAB1186) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using GGA2 antibody (CAB1186). Blue: DAPI for nuclear staining.

Antibodies

GGA2 Antibody (PACO09457)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)