Anti-GLMN Antibody (CAB12025)

Antibody Name:	Anti-GLMN Antibody
Antibody SKU:	CAB12025
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 405-594 of human GLMN (NP_444504.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	A-549, U-87MG, HepG2, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 405-594 of human GLMN (NP_444504.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RCLL NTSN HSGV EAFI IQNI KNQI DMSL KRTR NNKW FTGP QLIS LLDL VLFL PEGA ETDL LQNS DRIM ASLN LLRY LVIK DNEN DNQT GLWT ELGN IENN FLKP LHIG LNMS KAHY EAEI KNSQ EAQK SKDL CSIT VSGE EIPN MPPE MQLK VLHS ALFT FDLI ESVL ARVE ELIE IKTK STSE ENIG IK
Gene ID:	11146
Uniprot:	Q92990
Cellular Location:
Calculated MW:	48kDa/68kDa
Observed MW:	68kDa

Synonyms:	GLMN, FAP, FAP48, FAP68, FKBPAP, GLML, GVM, VMGLOM, glomulin
Background:	This gene encodes a phosphorylated protein that is a member of a Skp1-Cullin-F-box-like complex. The protein is essential for normal development of the vasculature and mutations in this gene have been associated with glomuvenous malformations, also called glomangiomas. Multiple splice variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	GLMN: Essential for normal development of the vasculature. May represent a naturally occurring ligand of the immunophilins FKBP59 and FKBP12. May function as an membrane anchoring protein. Isoform 1 may stimulate the p70S6K pathway. Isoform 2 may inhibit cell proliferation and increase IL2 production. Defects in GLMN are the cause of glomuvenous malformations (GVMs). GVMs are characterized by the presence of smooth-muscle-like glomus cells in the media surrounding distended vascular lumens. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cytokine Chromosomal Location of Human Ortholog: 1p22.1 Cellular Component: Cul2-RING ubiquitin ligase complex; cullin-RING ubiquitin ligase complex Molecular Function:hepatocyte growth factor receptor binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase inhibitor activity Biological Process: muscle cell differentiation; negative regulation of T cell proliferation; positive regulation of cytokine secretion; positive regulation of interleukin-2 biosynthetic process; positive regulation of phosphorylation; regulation of gene expression, epigenetic; regulation of proteasomal ubiquitin-dependent protein catabolic process; vasculogenesis Disease: Glomuvenous Malformations
NCBI Summary:	This gene encodes a phosphorylated protein that is a member of a Skp1-Cullin-F-box-like complex. The protein is essential for normal development of the vasculature and mutations in this gene have been associated with glomuvenous malformations, also called glomangiomas. Multiple splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016]
UniProt Code:	Q92990
NCBI GenInfo Identifier:	38372884
NCBI Gene ID:	11146
NCBI Accession:	Q92990.2
UniProt Secondary Accession:	Q92990,Q5VVC3, Q9BVE8,
UniProt Related Accession:	Q92990
Molecular Weight:	48,166 Da
NCBI Full Name:	Glomulin
NCBI Synonym Full Names:	glomulin, FKBP associated protein
NCBI Official Symbol:	GLMN
NCBI Official Synonym Symbols:	FAP; GVM; GLML; FAP48; FAP68; FKBPAP; VMGLOM
NCBI Protein Information:	glomulin
UniProt Protein Name:	Glomulin
UniProt Synonym Protein Names:	FK506-binding protein-associated protein; FAP; FKBP-associated protein
Protein Family:	Glomulin
UniProt Gene Name:	GLMN
UniProt Entry Name:	GLMN_HUMAN

Western blot analysis of extracts of various cell lines, using GLMN antibody (CAB12025) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)