The GLRX3 Antibody (CAB5892) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it a reliable choice for Western blot applications. By binding to the Glutaredoxin 3 protein, researchers can accurately analyze its expression and function in various cell types.Glutaredoxin 3 is known for its role in regulating the cellular redox state by facilitating the reduction of disulfide bonds in proteins. Dysregulation of this enzyme has been linked to oxidative stress-related diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
GLRX3 Antibody
SKU:
CAB5892
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GRX3, GRX4, GLRX4, PICOT, TXNL2, TXNL3, GLRX3
Positive Sample:
Raji, MCF-7, A-431, HeLa
Cellular Localization:
Cytoplasm, Z Line, Cell Cortex, Myofibril, Sarcomere.
Calculated MW:
37kDa
Observed MW:
37kDa
This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene.
Purification Method
Affinity purification
Gene ID
10539
RRID
AB_2766640
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GLRX3 Rabbit pAb (CAB5892) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg GLRX3 antibody (CAB5892). Western blot was performed from the immunoprecipitate using GLRX3 antibody (CAB5892) at a dilution of 1:1000.
