Description
| Product Name: | Glucose Uptake Assay Kit - Fluorometric |
| Product Code: | MAES0012 |
| Product Size: | 96 Assays |
| Sample Type: | Cells |
| Assay Time: | 100 min |
| Instrument: | Fluorescence microplate reader |
| Sensitivity: | 0.02 nmol/µL |
| Detection Range: | 0.02-0.5 nmol/µL |
Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2- deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG- 6-phosphate (2-DG-6P). 2-DG-6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG-6P is proportional to 2-DG (or glucose) uptake by cells.
2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
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